Faculty of Applied Life Science, Nippon Veterinary and Life Science University, Musashino 180-8602, Tokyo, Japan.
Lett Appl Microbiol. 2023 Apr 3;76(4). doi: 10.1093/lambio/ovad043.
For the purpose of clarifying the relationship between pasteurization and inactivation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in Saccharomyces pastorianus cells induced by pressurized carbon dioxide microbubbles (CO2MB) treatment, a storage test of S. pastorianus cells after CO2MB treatment was conducted to ascertain their recovery, and the treatment condition in the inactivation of GAPDH in S. pastorianus cells by CO2MB was investigated. Each population of S. pastorianus for 48, 96, and 144 h at 25°C was decreased significantly by CO2MB treatment at 35°C for 3 min (MB35-3 and MB35-5) or at 40°C and 45°C for 1 and 3 min (MB40-1, MB40-3, and MB45-1). In the storage test, recovery of treated cells was not observed after storage for 144 h at 25°C. The denaturation of GAPDH in the S. pastorianus cells caused by the same treatment as the storage test was detected by using sodium dodecyl sulphate polyacrylamide gel electrophoresis. While the activities at MB35-1, MB35-3, and MB40-1 were significantly higher than those at non-treatment, and those at MB35-5, MB40-3, and MB45-1 were lower. Therefore, GAPDH denaturation, but not the activity, was associated with the inactivation of S. pastorianus cells.
为了阐明巴氏杀菌和加压二氧化碳微泡（CO2MB）处理诱导的酿酒酵母细胞中甘油醛-3-磷酸脱氢酶（GAPDH）失活之间的关系，对 CO2MB 处理后的酿酒酵母细胞进行了储存试验，以确定其恢复情况，并研究了 CO2MB 处理对酿酒酵母细胞中 GAPDH 失活的处理条件。在 35°C 下用 CO2MB 处理 3 分钟（MB35-3 和 MB35-5）或在 40°C 和 45°C 下处理 1 分钟和 3 分钟（MB40-1、MB40-3 和 MB45-1），可使 25°C 下储存 48、96 和 144 小时的酿酒酵母种群数量显著减少。在储存试验中，在 25°C 下储存 144 小时后未观察到处理细胞的恢复。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳检测到与储存试验相同处理条件下酿酒酵母细胞中 GAPDH 的变性。虽然在 MB35-1、MB35-3 和 MB40-1 中的活性明显高于未处理组，而在 MB35-5、MB40-3 和 MB45-1 中的活性则较低。因此，GAPDH 的变性而不是活性与酿酒酵母细胞的失活有关。
