大鼠血管紧张素原的纯化

Purification of rat angiotensinogen.

作者信息

Sernia C, Davis M, Thomas W G

出版信息

Prep Biochem. 1986;16(1):45-59. doi: 10.1080/00327488608062458.

Abstract

A method of purifying rat angiotensinogen in three chromatography steps with a yield 3-4 times better than previous methods is described. Using chromatofocusing media for two steps and DEAE-affigel blue for the third step it was possible to separate angiotensinogen into three major peaks with pI of 5.25 (peak B), 4.80 (peak C) and 4.50 (peak D). Peaks B and C were completely purified with recoveries of 12% and 17% and specific activities of 21.8 and 20.0 micrograms AI/mg protein respectively. Analytical SDS-PAGE showed a 53,000 dalton band in both peaks with additional 51,000 and 57,000 dalton bands in peak C.

摘要

本文描述了一种通过三步色谱法纯化大鼠血管紧张素原的方法，其产率比以前的方法高3至4倍。在两步中使用色谱聚焦介质，第三步使用DEAE-琼脂糖蓝，可以将血管紧张素原分离为三个主要峰，其等电点分别为5.25（峰B）、4.80（峰C）和4.50（峰D）。峰B和峰C被完全纯化，回收率分别为12%和17%，比活性分别为21.8和20.0微克AI/毫克蛋白质。分析型SDS-PAGE显示两个峰中均有一条53,000道尔顿的条带，峰C中还有额外的51,000和57,000道尔顿的条带。