Teklemariam Daniel M, Gailing Oliver, Siregar Iskandar Z, Amandita Fitri Yola, Moura Carina C M
Department of Forest Genetics and Forest Tree Breeding University of Göttingen 37077 Göttingen Germany.
Centre of Biodiversity and Sustainable Land Use University of Göttingen Göttingen Germany.
Ecol Evol. 2023 Apr 7;13(4):e9935. doi: 10.1002/ece3.9935. eCollection 2023 Apr.
The high diversity and limited floral information in tropical forests often pose a challenge for species identification. However, over the past decade, DNA barcoding has been employed in tropical forests, including Sumatran forests, to enhance floristic surveys. This technique facilitates the discrimination of morphologically similar species and addresses the limitations of conventional species identification, which relies on short-lived reproductive structures. This study aimed to evaluate the efficiency of , , and the combination of both chloroplast markers for species identification in Burseraceae by employing genetic distance and species tree inference. In this study, we collected 197 specimens representing 20 species from five genera of Burseraceae. The highest percentage of specimens' identification (36%) at the species level was obtained using , followed by (31%), and (7%). The dataset presented the highest interspecific divergence with a mean of 0.008. In addition, a lack of barcode gap was observed in both markers, suggesting potential limitations of the core barcodes for distinguishing Sumatran species within Burseraceae. The monophyly test confirmed five species as monophyletic using Bayesian species tree inferences for . Overall, our results demonstrate that outperforms in species identification of Burseraceae, whereas their combination did not enhance species delimitation. To improve the molecular species assignments of this family, future studies may consider including more DNA markers in conjuction with , and broadening the availability of reference sequences for species that have not yet been included in the databases. The outcomes of molecular species identification vary depending on the taxonomic group under investigation. Implementation of phylogenomics for species delimitation and diagnostic marker development is strongly recommended for tropical biodiversity assessments, especially for poorly studied clades.
热带森林中高度的多样性和有限的植物信息常常给物种鉴定带来挑战。然而，在过去十年里，DNA条形码技术已被应用于包括苏门答腊森林在内的热带森林，以加强植物区系调查。这项技术有助于区分形态相似的物种，并解决了依赖短暂生殖结构的传统物种鉴定方法的局限性。本研究旨在通过利用遗传距离和物种树推断，评估叶绿体标记 、 以及两者组合在橄榄科物种鉴定中的效率。在本研究中，我们收集了代表橄榄科五个属20个物种的197个标本。在物种水平上，使用 鉴定出的标本百分比最高（36%），其次是 （31%）， （7%）。 数据集的种间差异最大，平均值为0.008。此外，在这两个标记中均未观察到条形码间隙，这表明核心条形码在区分橄榄科苏门答腊物种方面可能存在局限性。单系性检验通过对 的贝叶斯物种树推断，确认了五个物种为单系。总体而言，我们的结果表明，在橄榄科物种鉴定中 优于 ，而它们的组合并未增强物种界定。为了改进该科的分子物种分类，未来的研究可能需要考虑结合 纳入更多的DNA标记，并拓宽数据库中尚未包含的物种的参考序列的可用性。分子物种鉴定的结果因所研究的分类群而异。强烈建议将系统发育基因组学应用于物种界定和诊断标记开发，以进行热带生物多样性评估，特别是对于研究较少的类群。
