Zhao Xin-Yu, Chen Hui, Zhao Bo, Song Yuan-Meng, Lu Meng-Qi, Cui Jian-Sheng, Zhang Lu-Lu, Li Shuang-Jiang
Pollution Prevention Biotechnology Laboratory of Hebei Province, School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China.
Huan Jing Ke Xue. 2023 Apr 8;44(4):2223-2233. doi: 10.13227/j.hjkx.202204266.
Due to their importance in human medicine, quinolones (QNs), as a typical class of antibiotics, are considered to be the "highest priority critically important antimicrobials" by the World Health Organization (WHO). In order to clarify the spatial-temporal variation and risk of QNs in soil, 18 representative topsoil samples were respectively collected in September 2020 (autumn) and June 2021 (summer). The contents of QNs antibiotics in soil samples were determined using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), and the ecological risk and resistance risk were calculated using the risk quotient method (RQ). The results showed that:① the average content of QNs decreased from autumn to summer (the average contents of QNs were 94.88 μg·kgin autumn and 44.46 μg·kg in summer); the highest values appeared in the middle area. ② The average proportion of silt was without change, whereas the average proportion of clay and sand was increased and decreased, respectively; the average contents of total phosphorus (TP), ammonia nitrogen (NH-N), and nitrate nitrogen (NO-N) also decreased. ③ The content of QNs was significantly correlated with soil particle size, nitrite nitrogen (NO-N), and nitrate nitrogen (NO-N) (<0.05). ④ The combined ecological risk of QNs showed high risk level (RQ>1), whereas the combined resistance risk of QNs showed medium risk level (0.1<RQ ≤ 1). In terms of seasonal variation, RQ showed a downward trend. Overall, the ecological risk and resistance risk of QNs in soil for Shijiazhuang City require further attention, and the risk control of antibiotics in soil should be strengthened in the future.
喹诺酮类药物（QNs）作为一类典型的抗生素，因其在人类医学中的重要性，被世界卫生组织（WHO）视为“最高优先级的极其重要的抗菌药物”。为了阐明土壤中喹诺酮类药物的时空变化及风险，于2020年9月（秋季）和2021年6月（夏季）分别采集了18个具有代表性的表层土壤样本。采用高效液相色谱 - 串联质谱法（HPLC - MS/MS）测定土壤样本中喹诺酮类抗生素的含量，并运用风险商数法（RQ）计算生态风险和抗性风险。结果表明：①喹诺酮类药物的平均含量从秋季到夏季呈下降趋势（秋季喹诺酮类药物的平均含量为94.88 μg·kg，夏季为44.46 μg·kg）；最高值出现在中部地区。②粉砂的平均占比无变化，而黏土和砂的平均占比分别增加和减少；总磷（TP）、氨氮（NH - N）和硝态氮（NO - N）的平均含量也下降。③喹诺酮类药物的含量与土壤粒径、亚硝酸盐氮（NO - N）和硝态氮（NO - N）显著相关（<0.05）。④喹诺酮类药物的综合生态风险显示为高风险水平（RQ>1），而喹诺酮类药物的综合抗性风险显示为中等风险水平（0.1<RQ≤1）。就季节变化而言，风险商数呈下降趋势。总体而言，石家庄市土壤中喹诺酮类药物的生态风险和抗性风险需要进一步关注，未来应加强土壤中抗生素的风险管控。
