Department of Human Movement Science, Graduate School, Incheon National University, Incheon, Republic of Korea.
Department of Life Science, College of Life Sciences and Bioengineering, Incheon National University, Incheon, Republic of Korea.
J Physiol Sci. 2023 Apr 11;73(1):6. doi: 10.1186/s12576-023-00864-6.
Exercise is an effective intervention to ameliorate metabolic diseases including obesity and insulin resistance, but the mechanisms involved in the metabolic amelioration have not yet been fully elucidated. This study aimed to determine whether AMPK-SIRT1-PGC-1α-FNDC5/Irisin-UCP1 expression is activated and whether metabolic dysfunction is ameliorated by chronic voluntary wheel running (VWR) in high-fat diet (HFD) induced obese mice. C57BL6J mice were randomly assigned into three groups at the age of 7 weeks for 10 weeks: normal chow diet (CON) group, HFD group, and HFD + VWR group. Chronic VWR ameliorates metabolic parameters and leads to increases in the expression of PGC-1α in the gastrocnemius muscle in HFD-induced obese mice. In contrast, the expression of AMPKα, SIRT1, and FNDC5, or circulating irisin levels did not lead to alteration. Improvement of metabolic health was partly mediated via PGC-1α expression by chronic VWR, but not FNDC5/Irisin pathway in HFD-induced obese mice.
运动是改善代谢性疾病（包括肥胖和胰岛素抵抗）的有效干预措施，但涉及代谢改善的机制尚未完全阐明。本研究旨在确定慢性自愿轮跑（VWR）是否会激活 AMPK-SIRT1-PGC-1α-FNDC5/Irisin-UCP1 表达，以及是否会改善高脂肪饮食（HFD）诱导的肥胖小鼠的代谢功能障碍。7 周龄的 C57BL6J 小鼠被随机分为三组，进行 10 周的实验：正常饲料（CON）组、高脂肪饮食（HFD）组和 HFD+VWR 组。慢性 VWR 改善了代谢参数，并导致 HFD 诱导的肥胖小鼠腓肠肌中 PGC-1α 的表达增加。相比之下，AMPKα、SIRT1 和 FNDC5 的表达或循环 Irisin 水平没有发生改变。慢性 VWR 改善代谢健康的部分机制是通过 PGC-1α 表达介导的，但不是通过 HFD 诱导的肥胖小鼠的 FNDC5/Irisin 途径。
