Mansouri Vahid, Arjmand Babak, Hamzeloo-Moghadam Maryam, Razzaghi Zahra, Khodadoost Mahmood, Rezaei Tavirani Mostafa, Rezaei Tavirani Majid, Ahmadzadeh Alireza
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2022 Dec 27;13:e76. doi: 10.34172/jlms.2022.76. eCollection 2022.
Due to widespread penetration of UV radiation in human life, the biological effect of UV radiation is studied through many investigations in the field of medicine. There are many assessments about UV radiation which are concerned with protein-protein interaction (PPI) network analysis. In the present study, a network analysis associated with the complementary evaluation of UV radiation on human primary melanocytes is presented. The gene expression profiles of the irradiated human primary melanocytes and the control cells were extracted from Gene Expression Omnibus (GEO) and were evaluated via PPI network analysis and action map assessment. 69 significant differentially expressed genes (DEGs) were included in the main component of the PPI network. Brain-derived neurotrophic factor (BDNF), SNAI1, and SOCS1 were highlighted as the top dysregulated and hub genes. Results indicate that BDNF and SNAI1 participate in the regulatory unit including the total hubs and top dysregulated genes. Considerable down-regulation of BDNF and up-regulation of SNAI1 as the two critical targeted genes by UV radiation are accompanied by gross alteration in cell functions.
由于紫外线辐射在人类生活中的广泛渗透，医学领域通过许多研究来探讨紫外线辐射的生物学效应。关于紫外线辐射有许多评估，涉及蛋白质-蛋白质相互作用（PPI）网络分析。在本研究中，提出了一种与紫外线辐射对人原代黑素细胞的补充评估相关的网络分析。从基因表达综合数据库（GEO）中提取受辐照的人原代黑素细胞和对照细胞的基因表达谱，并通过PPI网络分析和作用图谱评估进行评价。69个显著差异表达基因（DEG）包含在PPI网络的主要成分中。脑源性神经营养因子（BDNF）、SNAI1和SOCS1被突出显示为失调最严重的关键基因和枢纽基因。结果表明，BDNF和SNAI1参与了包括总枢纽基因和失调最严重的基因在内的调控单元。紫外线辐射作为两个关键靶基因，BDNF的显著下调和SNAI1的上调伴随着细胞功能的重大改变。
