Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, United States.
Center for Population Biology, University of California, Davis, CA, United States.
J Hered. 2023 Jun 22;114(4):395-403. doi: 10.1093/jhered/esad013.
Climate-driven changes in hydrological regimes are of global importance and are particularly significant in riparian ecosystems. Riparian ecosystems in California provide refuge to many native and vulnerable species within a xeric landscape. California Tetragnatha spiders play a key role in riparian ecosystems, serving as a link between terrestrial and aquatic elements. Their tight reliance on water paired with the widespread distributions of many species make them ideal candidates to better understand the relative role of waterways versus geographic distance in shaping the population structure of riparian species. To assist in better understanding population structure, we constructed a reference genome assembly for Tetragnatha versicolor using long-read sequencing, scaffolded with proximity ligation Omni-C data. The near-chromosome-level assembly is comprised of 174 scaffolds spanning 1.06 Gb pairs, with a scaffold N50 of 64.1 Mb pairs and BUSCO completeness of 97.6%. This reference genome will facilitate future study of T. versicolor population structure associated with the rapidly changing environment of California.
气候驱动的水文变化具有全球重要性，在河岸生态系统中尤为显著。加利福尼亚的河岸生态系统为干旱景观中的许多本地和脆弱物种提供了避难所。加利福尼亚 Tetragnatha 蜘蛛在河岸生态系统中发挥着关键作用，它们是陆地和水生元素之间的纽带。它们对水的紧密依赖，加上许多物种的广泛分布，使它们成为更好地理解水道相对于地理距离在塑造河岸物种种群结构中的相对作用的理想候选者。为了帮助更好地理解种群结构，我们使用长读测序技术为 Tetragnatha versicolor 构建了参考基因组组装，并使用邻近连接 Omni-C 数据进行了支架搭建。接近染色体水平的组装由 174 个支架组成，跨越 10.6 Gb 对，支架 N50 为 64.1 Mb 对，BUSCO 完整性为 97.6%。这个参考基因组将有助于未来研究与加利福尼亚快速变化的环境相关的 T. versicolor 种群结构。
