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猞猁参考基因组序列组装

A Reference Genome Assembly of the Bobcat, Lynx rufus.

机构信息

Department of Ecology and Evolutionary Biology, University of California, Los Angeles, CA 90095, USA.

Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.

出版信息

J Hered. 2022 Nov 30;113(6):615-623. doi: 10.1093/jhered/esac031.

Abstract

The bobcat (Lynx rufus) is a medium-sized carnivore well adapted to various environments and an indicator species for landscape connectivity. It is one of the 4 species within the extant Lynx genus in the family Felidae. Because of its broad geographic distribution and central role in food webs, the bobcat is important for conservation. Here we present a high-quality de novo genome assembly of a male bobcat located in Mendocino County, CA, as part of the California Conservation Genomics Project (CCGP). The assembly was generated using the standard CCGP pipeline from a combination of Omni-C and HiFi technologies. The primary assembly comprises 76 scaffolds spanning 2.4 Gb, represented by a scaffold N50 of 142 Mb, a contig N50 of 66.2 Mb, and a BUSCO completeness score of 95.90%. The bobcat genome will be an important resource for the effective management and conservation of this species and comparative genomics exploration.

摘要

短尾猫(Lynx rufus)是一种适应各种环境的中型食肉动物,也是景观连通性的指示物种。它是猫科动物中的 4 种现存的猞猁属物种之一。由于其广泛的地理分布和在食物网中的核心作用,短尾猫对保护至关重要。这里我们展示了一只位于加利福尼亚州门多西诺县的雄性短尾猫的高质量从头基因组组装,这是加利福尼亚保护基因组学项目(CCGP)的一部分。该组装是使用标准的 CCGP 管道从 Omni-C 和 HiFi 技术的组合生成的。主要组装由 76 个支架组成,跨越 24 亿碱基对,由支架 N50 为 142Mb、串联 N50 为 66.2Mb 和 BUSCO 完整性分数为 95.90%表示。短尾猫基因组将是有效管理和保护该物种以及比较基因组学探索的重要资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a85f/9709964/3aed89e24248/esac031f0001.jpg

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