Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin, 150030, PR China.
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, 311300, PR China.
Int J Syst Evol Microbiol. 2023 Apr;73(4). doi: 10.1099/ijsem.0.005776.
A novel endophytic actinomycete, designated strain NEAU-365, was isolated from the root of collected from Nanjing, Jiangsu, PR China. Comparative 16S rRNA gene sequencing showed that strain NEAU-365 belonged to the genus but clearly differs from the currently recognized species Z350 (99.31 %), NEAU-KD1 (98.82 %) and MUSC 201 (97.78 %). Phylogenetic tree analysis revealed that strain NEAU-365 clustered with the type strains of the genus . The genome size was 4.1 Mbp with a DNA G+C content of 71.2 mol%. Digital DNA-DNA hybridization and average nucleotide identity values between the genome sequence of strain NEAU-365 and those of Z350(27.5 and 84.0 %), NEAU-KD1(23.4 and 80.9 %) and MUSC 201(20.9 and 77.7 %) were below the recommended thresholds for species delineation. Cells were observed to be irregular cocci shaped. The cell wall contained ll-diaminopimelic acid and the whole-cell sugars were galactose and rhamnose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid and three unidentified phospholipids. The predominant menaquinone was detected as MK-9(H). The major fatty acids were C, C, C ω9 and 10-methyl C. On the basis of genotypic and phenotypic differences from members of the genus , a novel species, sp. nov. is proposed. The type strain is NEAU-365 (=CCTCC AA 2021033=JCM 35005).
一株新的内生放线菌，命名为 NEAU-365 株，从中国江苏南京采集的植物根部分离得到。比较 16S rRNA 基因序列表明，菌株 NEAU-365 属于 属，但与目前公认的种 Z350(99.31%)、NEAU-KD1(98.82%)和 MUSC 201(97.78%)明显不同。系统发育树分析表明，菌株 NEAU-365 与属的模式菌株聚类。基因组大小为 4.1 Mbp，G+C 含量为 71.2mol%。数字 DNA-DNA 杂交和 NEAU-365 株基因组序列与 Z350(27.5 和 84.0%)、NEAU-KD1(23.4 和 80.9%)和 MUSC 201(20.9 和 77.7%)的平均核苷酸同一性值均低于种界定的推荐阈值。细胞呈不规则球菌状。细胞壁含有 ll-二氨基庚二酸，全细胞糖为半乳糖和鼠李糖。极性脂由双磷脂酰甘油、磷脂酰甘油、磷酸甘油脂和三种未鉴定的磷脂组成。主要的menaquinone 是 MK-9(H)。主要脂肪酸为 C、C、C ω9 和 10-甲基 C。基于与属成员的表型和基因型差异，提出了一个新的种， sp. nov.。模式菌株为 NEAU-365(=CCTCC AA 2021033=JCM 35005)。
