Department of Epidemiology, Shanxi Medical University School of Public Health, Taiyuan, Shanxi 030001, PR China.
Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2233-2238. doi: 10.1099/ijsem.0.003979. Epub 2020 Feb 25.
Two Gram-staining-positive, catalase-positive, oxidase-negative, aerobic, non-motile, irregular rod-shaped bacterial strains (Z350 and Z527) were isolated from intestinal contents of plateau pika () from the Qinghai-Tibet Plateau, PR China. Results of phylogenetic analyses based on 16S rRNA gene sequences indicated that strain Z350 belongs to the genus (family ) but clearly differs from the currently recognized species DSM 101040 (98.4 % similarity) and DSM 27763 (97.4 %). Strain Z350 had a DNA G+C content of 70.7 mol% and shared 80.4 and 76.7 % average nucleotide identity values and 23.4 and 20.6 % DNA-DNA hybridization relatedness with DSM 101040 and DSM 27763, respectively. Further phylogenetic analyses based on 497 core genes indicated that our isolates were members of the genus but separated from all existing genera within the family . The major cellular fatty acids were C ω9 and 10-methyl C. The cell wall contained ll-diaminopimelic acid as the diamino acid, and rhamnose, ribose and glucose as whole cell-wall sugars. MK-9(H) was detected as the major menaquinone. Polar lipids present were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified phospholipid. Based on distinct differences in the genotypic and phenotypic data from the two species, a novel species, sp. nov., is proposed. The type strain is Z350 (=CGMCC 4.7464=DSM 106288).
从中国青藏高原的高原兔肠道内容物中分离到两株革兰氏阳性、过氧化氢酶阳性、氧化酶阴性、需氧、非运动、不规则杆状细菌菌株（Z350 和 Z527）。基于 16S rRNA 基因序列的系统发育分析结果表明，菌株 Z350 属于 属（科），但与目前公认的种 DSM 101040（98.4%相似性）和 DSM 27763（97.4%相似性）明显不同。菌株 Z350 的 DNA G+C 含量为 70.7 mol%，与 DSM 101040 和 DSM 27763 的平均核苷酸同一性值分别为 80.4%和 76.7%，DNA-DNA 杂交相关性分别为 23.4%和 20.6%。进一步基于 497 个核心基因的系统发育分析表明，我们的分离株是属的成员，但与该科内所有现有属都分离。主要细胞脂肪酸为 C ω9 和 10-甲基 C。细胞壁含有 ll-二氨基庚二酸作为二氨基酸，以及鼠李糖、核糖和葡萄糖作为全细胞壁糖。检测到 MK-9(H)作为主要的menaquinone。存在的极性脂是双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和一种未鉴定的磷脂。基于两个种的基因型和表型数据的明显差异，提出了一个新的种， sp. nov.。模式菌株为 Z350（=CGMCC 4.7464=DSM 106288）。
