Laboratory of Animal Physiology, Department of Zoology, School of Biology, Faculty of Science, Aristotle University of Thessaloniki, GR-54124, Thessaloniki, Greece.
Department of Animal Science, Faculty of Agricultural Sciences, University of Western Macedonia, GR-53100, Florina, Greece.
Mar Environ Res. 2023 Jun;188:105977. doi: 10.1016/j.marenvres.2023.105977. Epub 2023 Apr 7.
Recently, P. nobilis populations have suffered a tremendous reduction, with pathogens potentially playing a crucial role. Considering its highly endangered status, mechanisms leading to mass mortalities were examined in one or multiple pathogens infected populations. Thus, seasonal antioxidant enzymatic activities, hsp70 and catalase mRNA levels, were investigated in two different Greek populations, during mass mortality events in summer of 2020. Samples were collected from Fthiotis and Lesvos during February (TC 14 ± 1.2 and 15 ± 1 respectively), April (TC 18 ± 1.2 and 17 ± 1.3 respectively), and June (TC 24.5 ± 1.5 and 21.5 ± 1.5 respectively) 2020. In July of the same year (TC 26.5 ± 1.7 in Fthiotis and 24.5 ± 1.7 in Lesvos), no live specimens were found. All biochemical parameters and phylogenetic analysis suggest that pathogen infection increases P. nobilis sensitivity to water temperature, subsequently leading to mass mortality. The latter was obvious in Fthiotis individuals, in which Haplosporidium pinnae was also observed with Mycobacterium spp., compared to Lesvos individuals.
近年来，P. nobilis 种群数量急剧减少，病原体可能发挥了关键作用。考虑到其高度濒危的状况，研究了一种或多种病原体感染种群中导致大量死亡的机制。因此，在 2020 年夏季大规模死亡事件期间，对来自希腊两个不同种群的个体进行了季节性抗氧化酶活性、hsp70 和过氧化氢酶 mRNA 水平的研究。分别于 2020 年 2 月（TC 14±1.2 和 15±1）、4 月（TC 18±1.2 和 17±1.3）和 6 月（TC 24.5±1.5 和 21.5±1.5）从弗西奥蒂斯和莱斯沃斯采集样本。同年 7 月（TC 26.5±1.7 在弗西奥蒂斯和 24.5±1.7 在莱斯沃斯），未发现活体标本。所有生化参数和系统发育分析表明，病原体感染增加了 P. nobilis 对水温的敏感性，随后导致大量死亡。在弗西奥蒂斯个体中，这种情况更为明显，与莱斯沃斯个体相比，还观察到 Haplosporidium pinnae 与 Mycobacterium spp. 同时存在。
