College of Agriculture, Hunan Agricultural University, Hunan Branch of National Oilseed Crops Improvement Center, Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Changsha 410128, China.
Int J Mol Sci. 2023 Mar 27;24(7):6277. doi: 10.3390/ijms24076277.
A high oleic acid content is considered an essential characteristic in the breeding of high-quality rapeseed in China. Long-chain non-coding RNA (lncRNA) molecules play an important role in the plant's growth and its response to stress. To better understand the role of lncRNAs in regulating plant reproductive development, we analyzed whole-transcriptome and physiological data to characterize the dynamic changes in lncRNA expression during the four representative times of seed development of high- and low-oleic-acid rapeseed in three regions. We identified 21 and 14 lncRNA and mRNA modules, respectively. These modules were divided into three types related to region, development stages, and material. Next, we analyzed the key modules related to the oil content and the oleic acid, linoleic acid, and linolenic acid contents with physiological data and constructed the key functional network analysis on this basis. Genes related to lipid metabolism, such as () and (), were present in the co-expression network, suggesting that the effect of these genes on lipid metabolism might be embodied by the expression of these lncRNAs. Our results provide a fresh insight into region-, development-stage-, and material-biased changes in lncRNA expression in the seeds of . Some of these lncRNAs may participate in the regulatory network of lipid accumulation and metabolism, together with regulated genes. These results may help elucidate the regulatory system of lncRNAs in the lipid metabolism of high-oleic-acid rapeseed seeds.
高油酸含量被认为是中国优质油菜育种的一个重要特征。长链非编码 RNA（lncRNA）分子在植物的生长及其对胁迫的反应中发挥着重要作用。为了更好地了解 lncRNAs 在调控植物生殖发育中的作用，我们分析了全转录组和生理数据，以描述三个地区高油酸和低油酸油菜种子发育的四个代表性时期 lncRNA 表达的动态变化。我们分别鉴定了 21 个和 14 个 lncRNA 和 mRNA 模块。这些模块分为与区域、发育阶段和材料相关的三种类型。接下来，我们利用生理数据分析了与油含量以及油酸、亚油酸和亚麻酸含量相关的关键模块，并在此基础上构建了关键功能网络分析。与脂质代谢相关的基因，如 （）和 （），存在于共表达网络中，这表明这些基因对脂质代谢的影响可能体现在这些 lncRNAs 的表达上。我们的研究结果为油菜种子中 lncRNA 表达的区域、发育阶段和材料偏向性变化提供了新的见解。其中一些 lncRNAs 可能参与了脂质积累和代谢的调控网络，以及受调控的基因。这些结果可能有助于阐明高油酸油菜种子中 lncRNAs 在脂质代谢调控系统中的作用。
