Hunan Branch of National Oilseed Crops Improvement Center, Changsha, China.
College of Agriculture, Hunan Agricultural University, Changsha, China.
PLoS One. 2022 Aug 18;17(8):e0272798. doi: 10.1371/journal.pone.0272798. eCollection 2022.
To scientifically evaluate and utilize high-oleic acid rape germplasm resources and cultivate new varieties suitable for planting in the Hunan Province, 30 local high-oleic acid rape germplasms from Hunan were used as materials. The 12 personality indices of quality, yield, and resistance were comprehensively evaluated by variability, correlation, principal component, and cluster analyses. The results of variability showed that except for oleic acid, the lowest coefficient of variation was oil content, which was 0.06. Correlation analysis showed that oil content was positively correlated with main traits such as yield per plant and oleic acid, which could be used in the early screening of high-oleic rape germplasm. The results of principal component analysis showed that the 12 personality indicators were integrated into four principal components, and the cumulative contribution rate was 62.487%. The value of comprehensive coefficient 'F' was positively correlated with the first, second, and fourth principal components and negatively correlated with the third principal component. Cluster analysis showed that 30 high-oleic rape germplasms could be divided into four categories consisting of 9 (30%), 6 (20%), 7 (23%), and 8 (27%) high-oleic rape germplasms, each with the characteristics of "high disease resistance", "high yield", "high protein", and "more stability". This study not only provides a reference basis for high-oleic rape breeding but also provides a theoretical basis for their early screening.
为科学评价和利用高油酸油菜种质资源，培育适宜湖南种植的油菜新品种，以 30 份湖南地方高油酸油菜资源为材料，采用变异系数、相关性、主成分和聚类分析对其品质、产量和抗性 12 个性状指标进行综合评价。变异系数分析结果表明，除油酸外，油分的变异系数最小，为 0.06。相关性分析表明，含油量与单株产量和油酸等主要性状呈正相关，可用于高油酸油菜种质的早期筛选。主成分分析结果表明，12 个性状指标可综合为 4 个主成分，累计贡献率为 62.487%。综合系数“F”值与第一、二、四主成分呈正相关，与第三主成分呈负相关。聚类分析表明，30 份高油酸油菜资源可分为 4 类，分别有 9 份（30%）、6 份（20%）、7 份（23%）和 8 份（27%）高油酸油菜资源，分别具有“高抗病性”、“高产”、“高蛋白”和“更稳定”的特点。本研究不仅为高油酸油菜的选育提供了参考依据，也为其早期筛选提供了理论依据。
