Zhao Dongfang, Zhou Bingqian, Hong Bo, Mao Jiajun, Chen Hu, Wu Junjie, Liao Li, Guan Chunyun, Guan Mei
College of Agriculture, Hunan Agricultural University, Changsha 410128, China.
Hunan Branch of National Oilseed Crops Improvement Center, Changsha 410128, China.
Plants (Basel). 2025 Jan 13;14(2):202. doi: 10.3390/plants14020202.
Rapeseed ( L.) is one of the four major oilseed crops in the world and is rich in fatty acids. Changes in the fatty acid composition affect the quality of rapeseed. Fatty acids play various roles in plants, but the functions of the genes involved in the fatty acid composition during plant development remain unclear. β-Ketoacyl-CoA synthase (KCS) is a key enzyme involved in the elongation of fatty acids. Various types of fatty acid products are used to build lipid molecules, such as oils, suberin, wax, and membrane lipids. In , and belong to the KCS family, but their specific functions remain unclear. This study cloned and from L. and analyzed their functions. The gene structures of and were similar and they were localized to the endoplasmic reticulum (ER). In yeast, overexpression of increased the ratios of palmitoleic acid and oleic acid, while decreased the ratios of oleic acid. In Arabidopsis, overexpression of and lead to an increase in the proportion of linoleic acid and a decrease in the erucic acid. In summary, and altered the composition ratios of fatty acids. These findings lay the foundation for an understanding of the role of KCS in the fatty acids in rapeseed, potentially improving its health and nutritional qualities.
油菜（L.）是世界四大油料作物之一，富含脂肪酸。脂肪酸组成的变化会影响油菜籽的品质。脂肪酸在植物中发挥着多种作用，但在植物发育过程中参与脂肪酸组成的基因功能仍不清楚。β-酮脂酰-CoA合酶（KCS）是参与脂肪酸延长的关键酶。各种类型的脂肪酸产物用于构建脂质分子，如油、木栓质、蜡和膜脂。在 、 和 中属于KCS家族，但其具体功能仍不清楚。本研究从油菜L.中克隆了 和 并分析了它们的功能。 和 的基因结构相似，且它们定位于内质网（ER）。在酵母中， 的过表达增加了棕榈油酸和油酸的比例，而 降低了油酸的比例。在拟南芥中， 和 的过表达导致亚油酸比例增加，芥酸比例降低。总之， 和 改变了脂肪酸的组成比例。这些发现为理解KCS在油菜籽脂肪酸中的作用奠定了基础，有可能改善其健康和营养品质。
