Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Xiamen Key Laboratory of Synthetic Biotechnology, Xiamen University, Xiamen 361005, China.
Int J Mol Sci. 2023 Mar 29;24(7):6396. doi: 10.3390/ijms24076396.
Organic solvent tolerant oxidoreductases are significant for both scientific research and biomanufacturing. However, it is really challenging to obtain oxidoreductases due to the shortages of natural resources and the difficulty to obtained it via protein modification. This review summarizes the recent advances in gene mining and structure-functional study of oxidoreductases from extremophiles for non-aqueous reaction systems. First, new strategies combining genome mining with bioinformatics provide new insights to the discovery and identification of novel extreme oxidoreductases. Second, analysis from the perspectives of amino acid interaction networks explain the organic solvent tolerant mechanism, which regulate the discrete structure-functional properties of extreme oxidoreductases. Third, further study by conservation and co-evolution analysis of extreme oxidoreductases provides new perspectives and strategies for designing robust enzymes for an organic media reaction system. Furthermore, the challenges and opportunities in designing biocatalysis non-aqueous systems are highlighted.
有机溶剂耐受氧化还原酶在科学研究和生物制造中都具有重要意义。然而，由于自然资源的短缺以及通过蛋白质修饰获得它的困难，获得氧化还原酶确实具有挑战性。本综述总结了近年来从极端微生物中获得非水反应系统中氧化还原酶的基因挖掘和结构-功能研究的最新进展。首先，将基因组挖掘与生物信息学相结合的新策略为发现和鉴定新型极端氧化还原酶提供了新的思路。其次，从氨基酸相互作用网络的角度进行分析，解释了有机溶剂耐受机制，调节了极端氧化还原酶离散的结构-功能特性。第三，通过对极端氧化还原酶的保守性和共进化分析的进一步研究，为设计用于有机介质反应系统的稳健酶提供了新的视角和策略。此外，还强调了设计非水相生物催化体系的挑战和机遇。
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