Department of Pathology, College of Medicine, Soonchunhyang University, 31 Soonchunhyang 6 gil, Dongnam-gu, Cheonan 31151, Chungcheongnam-do, Republic of Korea.
Department of Oncology, College of Medicine, Soonchunhyang University, 31 Soonchunhyang 6 gil, Dongnam-gu, Cheonan 31151, Chungcheongnam-do, Republic of Korea.
Int J Mol Sci. 2023 Mar 30;24(7):6476. doi: 10.3390/ijms24076476.
Cancer is a major disease and the leading cause of death worldwide, with colorectal cancer (CRC) being the third-most common cancer in Korea. The survival rate associated with CRC reduces as the disease stage increases. Therefore, its early detection and treatment can greatly increase patient survival rates. In this study, we identified the tetraspanin 5 () gene as an important biomarker for predicting the prognosis of patients with CRC. A TMA slide was used for statistical analysis. pN and clinical stage were found to be significant factors according to chi-square analysis, whereas pT, pN, metastasis, clinical stage, and TSPAN5 expression were significant according to Cox regression analysis. In order to prove the usefulness of TSPAN5, which is overexpressed in patients with metastatic CRC, as a biomarker, proliferation, migration, invasion, and tumorigenicity were examined using cell lines inhibited using small interfering RNA. The evaluations confirmed that suppression, in turn, suppressed proliferation, migration, invasion, and tumorigenesis, which are characteristic of cancer cells. Therefore, the evaluation of TSPAN5 expression may help observe the prognosis of CRC and determine an appropriate treatment method for patients with CRC.
癌症是一种主要疾病，也是全球范围内的主要死亡原因，结直肠癌（CRC）是韩国第三常见的癌症。CRC 的疾病分期越高，与之相关的生存率越低。因此，早期发现和治疗可以大大提高患者的生存率。在这项研究中，我们确定了四跨膜蛋白 5（TSPAN5）基因是预测 CRC 患者预后的重要生物标志物。使用 TMA 幻灯片进行统计分析。根据卡方分析，pN 和临床分期是显著相关的因素，而 Cox 回归分析显示 pT、pN、转移、临床分期和 TSPAN5 表达也是显著相关的因素。为了证明在转移性 CRC 患者中过表达的 TSPAN5 作为生物标志物的有用性，使用小干扰 RNA 抑制的细胞系进行了增殖、迁移、侵袭和致瘤性检测。评估结果证实，抑制 TSPAN5 会抑制增殖、迁移、侵袭和肿瘤发生，这些都是癌细胞的特征。因此，评估 TSPAN5 的表达可能有助于观察 CRC 的预后，并为 CRC 患者确定适当的治疗方法。
