The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, China.
College of Tea Sciences, Institute of Plant Health & Medicine, Guizhou University, Guiyang 550025, China.
Int J Mol Sci. 2023 Mar 30;24(7):6480. doi: 10.3390/ijms24076480.
Brassinosteroids (BRs) are important hormones that play crucial roles in plant growth, reproduction, and responses to abiotic and biotic stresses. CYP85A1 is a castasterone (CS) synthase that catalyzes C-6 oxidation of 6-deoxocastasterone (6-deoxoCS) to CS, after which CS is converted into brassinolide (BL) in a reaction catalyzed by CYP85A2. Here, we report the functional characteristics of rice ( L.) . Constitutive expression of driven by the cauliflower mosaic virus 35S promoter increased endogenous BR levels and significantly promoted growth and biomass production in three groups of transgenic lines. The plant height and stem diameter of the transgenic poplar plants were increased by 17.6% and 33.6%, respectively, in comparison with control plants. Simultaneously, we showed that expression of enhanced xylem formation in transgenic poplar without affecting cell wall thickness or the composition of cellulose. Our findings suggest that represents a potential target candidate gene for engineering fast-growing trees with improved wood production.
油菜素内酯（BRs）是一类重要的植物激素，在植物的生长、繁殖以及应对非生物和生物胁迫方面发挥着至关重要的作用。CYP85A1 是一种油菜素内酯（CS）合酶，能够催化 6-去氧白菜醇（6-deoxoCS）的 C-6 位氧化，生成 CS，然后 CS 在 CYP85A2 催化的反应中转化为油菜素内酯（BL）。在这里，我们报告了水稻（L.）的功能特征。组成型表达的油菜素内酯合成酶基因受花椰菜花叶病毒 35S 启动子的驱动，可增加内源油菜素内酯水平，并显著促进三组转基因水稻株系的生长和生物量的产生。与对照植株相比，转基因杨树植株的株高和茎直径分别增加了 17.6%和 33.6%。同时，我们表明表达的油菜素内酯合成酶基因增强了转基因杨树的木质部形成，而不影响细胞壁厚度或纤维素的组成。我们的研究结果表明，代表了一个潜在的目标候选基因，可用于工程改良木材生产的速生树木。
