Key Laboratory of Mass Spectrometry Imaging and Metabolomics (Minzu University of China), National Ethnic Affairs Commission, Beijing 100081, China.
Key Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing 100081, China.
Int J Mol Sci. 2023 Mar 30;24(7):6502. doi: 10.3390/ijms24076502.
Long non-coding RNAs (lncRNAs) have been shown to play critical regulatory roles in plants. can survive under severe low-temperature stress, and lncRNAs may play crucial roles in the gene regulation network underlying the cold stress response in . To investigate the roles of lncRNAs in the cold stress response of , a combined lncRNA and mRNA expression profiling under cold stress was conducted. Up to 4890 novel lncRNAs were identified in and 1322 of them were differentially expressed under cold stress, including 543 up-regulated and 779 down-regulated lncRNAs. A total of 421 lncRNAs were found to participate in the cold stress response by forming lncRNA-mRNA modules and regulating the genes encoding the stress-related transcription factors and enzymes in a cis-acting manner. We found that 31 lncRNAs acting as miRNA precursors and 8 lncRNAs acting as endogenous competitive targets of miRNAs participated in the cold stress response by forming lncRNA-miRNA-mRNA regulatory modules. In particular, a cold stress-responsive lncRNA, , which was experimentally proven to be an endogenous competitive target of , contributed to the cold stress adaptation by regulating in . These results provide new data for understanding the biological roles of lncRNAs in response to cold stress in plants.
长非编码 RNA（lncRNA）在植物中发挥着关键的调控作用。拟南芥可以在严重的低温胁迫下存活，lncRNA 可能在低温胁迫响应的基因调控网络中发挥重要作用。为了研究 lncRNA 在拟南芥低温胁迫响应中的作用，对低温胁迫下的 lncRNA 和 mRNA 表达谱进行了联合分析。在拟南芥中鉴定出了多达 4890 个新的 lncRNA，其中 1322 个在低温胁迫下差异表达，包括 543 个上调和 779 个下调的 lncRNA。共有 421 个 lncRNA 通过形成 lncRNA-mRNA 模块，以顺式作用的方式调节与应激相关的转录因子和酶的编码基因，参与到低温胁迫响应中。我们发现，31 个作为 miRNA 前体的 lncRNA 和 8 个作为 miRNA 内源性竞争靶标的 lncRNA 通过形成 lncRNA-miRNA-mRNA 调控模块参与到低温胁迫响应中。特别是一个冷响应的 lncRNA，，它被实验证明是 的内源性竞争靶标，通过调节 在低温胁迫适应中发挥作用。这些结果为理解 lncRNA 在植物低温胁迫响应中的生物学功能提供了新的数据。
