Song Giha, Son Seungmin, Nam Suhyeon, Suh Eun-Jung, Lee Soo In, Park Sang Ryeol
National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of Korea.
Department of Crop Science & Biotechnology, Jeonbuk National University, Jeonju 54896, Republic of Korea.
Int J Mol Sci. 2023 Apr 1;24(7):6604. doi: 10.3390/ijms24076604.
Every year, invasive pathogens cause significant damage to crops. Thus, identifying genes conferring broad-spectrum resistance to invading pathogens is critical for plant breeding. We previously demonstrated that OsWRKY114 contributes to rice ( L.) immunity against the bacterial pathovar pv. (). However, it is not known whether OsWRKY114 is involved in defense responses to other pathogens. In this study, we revealed that OsWRKY114 enhances innate immunity in rice against the fungal pathogen , which is the causal agent of bakanae disease. Transcript levels of various gibberellin-related genes that are required for plant susceptibility to were reduced in rice plants overexpressing . Analysis of disease symptoms revealed increased innate immunity against in -overexpressing rice plants. Moreover, the expression levels of genes, which encode negative regulators of jasmonic acid signaling that confer immunity against , were reduced in -overexpressing rice plants. These results indicate that OsWRKY114 confers broad-spectrum resistance not only to but also to . Our findings provide a basis for developing strategies to mitigate pathogen attack and improve crop resilience to biotic stress.
每年，入侵性病原体都会对农作物造成重大损害。因此，鉴定赋予对入侵病原体广谱抗性的基因对于植物育种至关重要。我们之前证明了OsWRKY114有助于水稻对细菌性致病型pv.（）的免疫。然而，尚不清楚OsWRKY114是否参与对其他病原体的防御反应。在本研究中，我们揭示了OsWRKY114增强了水稻对引起恶苗病的真菌病原体的先天免疫。在过表达的水稻植株中，植物对敏感所需的各种赤霉素相关基因的转录水平降低。对疾病症状的分析表明，过表达的水稻植株对的先天免疫增强。此外，在过表达的水稻植株中，编码赋予对免疫的茉莉酸信号负调控因子的基因的表达水平降低。这些结果表明，OsWRKY114不仅赋予对的广谱抗性，也赋予对的广谱抗性。我们的研究结果为制定减轻病原体攻击和提高作物对生物胁迫恢复力的策略提供了基础。
