Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China.
School of Materials Science and Engineering, Central South University, Changsha 410013, China.
Int J Mol Sci. 2023 Apr 3;24(7):6696. doi: 10.3390/ijms24076696.
The cryopreservation of red blood cells (RBCs) holds great potential for ensuring timely blood transfusions and maintaining an adequate RBC inventory. The conventional cryoprotectants (CPAs) have a lot of limitations, and there is an obvious need for novel, efficient, and biocompatible CPAs. Here, it is shown for the first time that the addition of dimethylglycine (DMG) improved the thawed RBC recovery from 11.55 ± 1.40% to 72.15 ± 1.22%. We found that DMG could reduce the mechanical damage by inhibiting ice formation and recrystallization during cryopreservation. DMG can also scavenge reactive oxygen species (ROS) and maintain endogenous antioxidant enzyme activities to decrease oxidative damage during cryopreservation. Furthermore, the properties of thawed RBCs were found to be similar to the fresh RBCs in the control. Finally, the technique for order performance by similarity to ideal solution (TOPSIS) was used to compare the performance of glycerol (Gly), hydroxyethyl starch (HES), and DMG in cryopreservation, and DMG exhibited the best efficiency. This work confirms the use of DMG as a novel CPA for cryopreservation of RBCs and may promote clinical transfusion therapy.
红细胞（RBC）的冷冻保存具有很大的潜力，可以确保及时输血和维持足够的 RBC 库存。传统的冷冻保护剂（CPAs）有很多局限性，因此需要新型、高效、生物相容的 CPAs。本研究首次表明，添加二甲甘氨酸（DMG）可将解冻后的 RBC 回收率从 11.55±1.40%提高到 72.15±1.22%。研究发现，DMG 可以通过抑制冷冻保存过程中的冰晶形成和重结晶来减少机械损伤。DMG 还可以清除活性氧（ROS）并维持内源性抗氧化酶活性，以减少冷冻保存过程中的氧化损伤。此外，发现解冻后的 RBC 性质与对照组中的新鲜 RBC 相似。最后，采用理想溶液相似排序法（TOPSIS）比较甘油（Gly）、羟乙基淀粉（HES）和 DMG 在冷冻保存中的性能，结果表明 DMG 效果最佳。本研究证实了 DMG 作为一种新型 RBC 冷冻保护剂的用途，可能会促进临床输血治疗。
