Institute for Pharmaceutical and Biomedical Sciences, Johannes Gutenberg-University, 55128 Mainz, Germany.
Institute of Human Genetics, University Medical Center Mainz, 55131 Mainz, Germany.
Cells. 2023 Mar 30;12(7):1051. doi: 10.3390/cells12071051.
In the last decade, we have witnessed an upsurge in nuclei-based studies, particularly coupled with next-generation sequencing. Such studies aim at understanding the molecular states that exist in heterogeneous cell populations by applying increasingly more affordable sequencing approaches, in addition to optimized methodologies developed to isolate and select nuclei. Although these powerful new methods promise unprecedented insights, it is important to understand and critically consider the associated challenges. Here, we provide a comprehensive overview of the rise of nuclei-based studies and elaborate on their advantages and disadvantages, with a specific focus on their utility for transcriptomic sequencing analyses. Improved designs and appropriate use of the various experimental strategies will result in acquiring biologically accurate and meaningful information.
在过去的十年中，我们见证了基于核的研究的兴起，特别是与下一代测序相结合的研究。这些研究旨在通过应用越来越实惠的测序方法，以及开发的用于分离和选择核的优化方法，来理解异质细胞群体中存在的分子状态。尽管这些强大的新方法承诺提供前所未有的见解，但重要的是要理解并批判性地考虑相关的挑战。在这里，我们提供了基于核的研究兴起的全面概述，并详细阐述了它们的优缺点，特别关注它们在转录组测序分析中的实用性。改进设计和适当使用各种实验策略将导致获得生物学上准确和有意义的信息。
