McReynolds Colin, Adrien Amandine, Silvestre de Ferron Antoine, Boussetta Nadia, Grimi Nabil, Pecastaing Laurent, Fernandes Susana C M
IPREM-Institute of Analytical Sciences and Physico-Chemistry for Environment and Materials, E2S UPPA: Energy Environment Solutions, Université de Pau et des Pays de l'Adour, CNRS-Centre National de la Recherche Scientifique, 64600 Anglet, France.
MANTA-Marine Materials Research Group, Universite de Pau et des Pays de l'Adour, E2S UPPA, 64600 Anglet, France.
Foods. 2023 Mar 30;12(7):1473. doi: 10.3390/foods12071473.
(syn. ) is an industrially and ecologically important species of red alga used for the production of high-quality agar. However, the species is also of growing interest for the production of other valuable compounds, such as mycosporine-like amino acids (MAAs), with potential cosmeceutical and biomedical applications. Novel methods using two pulsed power techniques, high-voltage electrical discharges (HVED) and pulsed electrical fields (PEF), were evaluated for efficacy of MAA extraction. Algal suspensions were prepared at two ratios (1:20 and 1:40 :). Four different extraction protocols were compared: (i) high-voltage electrical discharges, (ii) pulsed electric fields, (iii) maceration at room temperature, and (iv) maceration at 50 °C. The algae were treated in three states: freshly harvested, dried, and powdered. HVED and PEF treatments were effective when performed on fresh algae, and in particular the HVED treatment resulted in yields of MAAs twenty times higher than the control: 0.81 ± 0.05 mg/g vs. 0.037 ± 0.002 mg/g. This effect was not observed to the same extent when the algae were dried or powdered, although HVED remained the most selective method overall.
（同义词）是一种在工业和生态方面都很重要的红藻，用于生产高质量的琼脂。然而，该物种在生产其他有价值的化合物方面也越来越受到关注，例如具有潜在药妆和生物医学应用的类菌孢素氨基酸（MAA）。评估了使用两种脉冲功率技术，即高压放电（HVED）和脉冲电场（PEF）提取MAA的新方法的有效性。以两种比例（1:20和1:40）制备藻类悬浮液。比较了四种不同的提取方案：（i）高压放电，（ii）脉冲电场，（iii）室温浸渍，以及（iv）50℃浸渍。藻类以三种状态进行处理：新鲜收获、干燥和粉末状。对新鲜藻类进行HVED和PEF处理是有效的，特别是HVED处理使MAA的产量比对照高二十倍：0.81±0.05毫克/克对0.037±0.002毫克/克。当藻类干燥或呈粉末状时，这种效果没有在相同程度上观察到，尽管总体而言HVED仍然是最具选择性的方法。
