Smirnov Aleksei N, Aslanov Simar F, Danilov Denis V, Kurapova Olga Yu, Solovyeva Elena V
Chemistry Institute, Saint-Petersburg State University, 26 Universitetsky Pr., Peterhof, 198504 Saint-Petersburg, Russia.
Interdisciplinary Center for Nanotechnology, Saint-Petersburg State University, 1 Ulianovskaya Str., Peterhof, 198504 Saint-Petersburg, Russia.
Nanomaterials (Basel). 2023 Apr 3;13(7):1267. doi: 10.3390/nano13071267.
Anisotropic gold nanoparticles have been recognized as promising agents for medical diagnostics and cancer therapy due to their wide functionality, photothermal effect, and ability for optical signal amplification in the near-infrared range. In this work, a simple and rapid method for the preparation of bone-shaped gold nanoparticles coated with a dye-impregnated silica shell with an aminated surface is proposed. The possibility of further functionalization the nanostructures with a delivery vector using folic acid as an example is demonstrated. The average size of the resulting tags does not exceed 70 nm, meeting the criteria of cell endocytosis. The prepared tags exhibit surface-enhanced Raman scattering (SERS) spectra at excitation with lasers of 632.8 and 785 nm. Cell imaging is performed on HeLa cells based on the most pronounced SERS bands as a tracking signal. The obtained images, along with scanning electron microscopy of cell samples, revealed the tendency of tags to agglomerate during endocytosis followed by the "hot spots" effect. To evaluate the toxic and proliferative effect of the nanotags, an MTT assay was performed with two HeLa and HEP G2 cell lines. The results revealed higher viability for HEP G2 cells.
各向异性金纳米颗粒因其广泛的功能、光热效应以及在近红外范围内进行光信号放大的能力，已被公认为是用于医学诊断和癌症治疗的有前景的试剂。在这项工作中，提出了一种简单快速的方法来制备涂覆有染料浸渍二氧化硅壳且表面胺化的骨形金纳米颗粒。以叶酸为例，展示了使用递送载体对纳米结构进行进一步功能化的可能性。所得标签的平均尺寸不超过70 nm，符合细胞内吞作用的标准。制备的标签在632.8和785 nm激光激发下呈现表面增强拉曼散射（SERS）光谱。基于最明显的SERS谱带作为跟踪信号，对HeLa细胞进行细胞成像。获得的图像以及细胞样品的扫描电子显微镜显示，标签在胞吞作用期间有聚集趋势，随后产生“热点”效应。为了评估纳米标签的毒性和增殖作用，对两种HeLa和HEP G2细胞系进行了MTT试验。结果显示HEP G2细胞具有更高的活力。
