Arsalani Saeideh, Arsalani Soudabeh, Isikawa Mileni, Guidelli Eder J, Mazon Ernesto E, Ramos Ana Paula, Bakuzis Andris, Pavan Theo Z, Baffa Oswaldo, Carneiro Antonio A O
Department of Physics, FFCLRP, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto 14040-901, São Paulo, Brazil.
Physikalisch-Technische Bundesanstalt, Abbestrasse 2-12, D-10587 Berlin, Germany.
Nanomaterials (Basel). 2023 Jan 20;13(3):434. doi: 10.3390/nano13030434.
The development of nanomaterials has drawn considerable attention in nanomedicine to advance cancer diagnosis and treatment over the last decades. Gold nanorods (GNRs) and magnetic nanoparticles (MNPs) have been known as commonly used nanostructures in biomedical applications due to their attractive optical properties and superparamagnetic (SP) behaviors, respectively. In this study, we proposed a simple combination of plasmonic and SP properties into hybrid NPs of citrate-coated manganese ferrite (Ci-MnFeO) and cetyltrimethylammonium bromide-coated GNRs (CTAB-GNRs). In this regard, two different samples were prepared: the first was composed of Ci-MnFeO (0.4 wt%), and the second contained hybrid NPs of Ci-MnFeO (0.4 wt%) and CTAB-GNRs (0.04 wt%). Characterization measurements such as UV-Visible spectroscopy and transmission electron microscopy (TEM) revealed electrostatic interactions caused by the opposing surface charges of hybrid NPs, which resulted in the formation of small nanoclusters. The performance of the two samples was investigated using magneto-motive ultrasound imaging (MMUS). The sample containing Ci-MnFeO_CTAB-GNRs demonstrated a displacement nearly two-fold greater than just using Ci-MnFeO; therefore, enhancing MMUS image contrast. Furthermore, the preliminary potential of these hybrid NPs was also examined in magnetic hyperthermia (MH) and photoacoustic imaging (PAI) modalities. Lastly, these hybrid NPs demonstrated high stability and an absence of aggregation in water and phosphate buffer solution (PBS) medium. Thus, Ci-MnFeO_CTAB-GNRs hybrid NPs can be considered as a potential contrast agent in MMUS and PAI and a heat generator in MH.
在过去几十年中，纳米材料的发展在纳米医学领域引起了广泛关注，以推动癌症诊断和治疗的进步。金纳米棒（GNRs）和磁性纳米颗粒（MNPs）因其分别具有吸引人的光学特性和超顺磁性（SP）行为，而被认为是生物医学应用中常用的纳米结构。在本研究中，我们提出了一种将等离子体和SP特性简单结合到柠檬酸盐包覆的锰铁氧体（Ci-MnFeO）和十六烷基三甲基溴化铵包覆的GNRs（CTAB-GNRs）的混合纳米颗粒中的方法。在这方面，制备了两种不同的样品：第一种由Ci-MnFeO（0.4 wt%）组成，第二种包含Ci-MnFeO（0.4 wt%）和CTAB-GNRs（0.04 wt%）的混合纳米颗粒。诸如紫外可见光谱和透射电子显微镜（TEM）等表征测量揭示了混合纳米颗粒相反表面电荷引起的静电相互作用，这导致了小纳米团簇的形成。使用磁动力超声成像（MMUS）研究了这两种样品的性能。含有Ci-MnFeO_CTAB-GNRs的样品表现出的位移几乎是仅使用Ci-MnFeO时的两倍；因此，增强了MMUS图像对比度。此外，还在磁热疗（MH）和光声成像（PAI）模式下研究了这些混合纳米颗粒的初步潜力。最后，这些混合纳米颗粒在水和磷酸盐缓冲溶液（PBS）介质中表现出高稳定性且无聚集现象。因此，Ci-MnFeO_CTAB-GNRs混合纳米颗粒可被视为MMUS和PAI中的潜在造影剂以及MH中的发热体。
