Hunan Province Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China.
National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Changsha 410128, China.
Nutrients. 2023 Mar 31;15(7):1725. doi: 10.3390/nu15071725.
To explore the mechanism by which cell-free supernatant improves glucose and lipid metabolisms in , the present study used different dilution concentrations of cell-free supernatant as an intervention for with under a high-glucose diet. The changes in lifespan, exercise ability, level of free radicals, and characteristic indexes of glucose and lipid metabolisms were studied. Furthermore, the expression of key genes of glucose and lipid metabolisms was detected by qRT-PCR. The results showed that cell-free supernatant significantly improved the movement ability, prolonged the lifespan, reduced the level of ROS, and alleviated oxidative damage in . cell-free supernatant supported resistance to increases in glucose and triglyceride induced by a high-glucose diet and downregulated the expression of key genes of glucose metabolism, such as , , , and while upregulating the expression of key genes of lipid metabolism, such as , , , and as well as down-regulating the expression of the gene to inhibit fatty acid biosynthesis. These findings indicated that cell-free supernatant, as a postbiotic, has the potential to prevent obesity and improve glucose metabolism disorders and other diseases.
为了探索细胞外上清液改善 的葡萄糖和脂代谢的机制，本研究采用不同稀释浓度的细胞外上清液作为高糖饮食下 的干预物。研究了寿命、运动能力、自由基水平以及葡萄糖和脂代谢特征指标的变化。此外，通过 qRT-PCR 检测了葡萄糖和脂代谢关键基因的表达。结果表明，细胞外上清液显著提高了 的运动能力，延长了寿命，降低了 ROS 水平，并减轻了 的氧化损伤。细胞外上清液支持抵抗高糖饮食引起的葡萄糖和甘油三酯升高，并下调葡萄糖代谢关键基因的表达，如 、 、 、 ，同时上调脂代谢关键基因的表达，如 、 、 、 ，以及下调 基因的表达以抑制脂肪酸合成。这些发现表明，细胞外上清液作为后生元，有可能预防肥胖和改善葡萄糖代谢紊乱等疾病。
