School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, Kazakhstan.
Higher School of Medicine, Al-Farabi Kazakh National University, Tole-bi 96, Almaty 050040, Kazakhstan.
Molecules. 2023 Apr 4;28(7):3210. doi: 10.3390/molecules28073210.
Numerous natural habitats, such as soil, air, fermented foods, and human stomachs, are home to different strains. Some strains have a distinctive predominance and are widely recognized among other microbial communities, as a result of their varied habitation and physiologically active metabolites. The present study collected vegetable products (potato, carrot, and tomato) from local markets in Almaty, Kazakhstan. The bacterial isolates were identified using biochemical and phylogenetic analyses after culturing. Our phylogenetic analysis revealed three Gram-positive bacterial isolates BSS11, BSS17, and BSS19 showing 99% nucleotide sequence similarities with O-3, Md1-42, and Khozestan2. The crude extract was prepared from bacterial isolates to assess the antibiotic resistance potency and the antimicrobial potential against various targeted multidrug-resistant strains, including , , , , , , , , , , , , , , and . This study found that the species that were identified have the ability to produce antibiotic chemicals. Additionally, the GC-MS analysis of three bacterial extracts revealed the presence of many antibiotic substances including phenol, benzoic acid, 1,2-benzenedicarboxylic acid and bis(2-methylpropyl), methoxyphenyl-oxime, and benzaldehyde. This work sheds light on the potential of to be employed as an antimicrobial agent to target different multidrug-resistant bacterial strains. The results indicate that market vegetables may be a useful source of strains displaying a range of advantageous characteristics that can be used in the creation of biological antibiotics.
许多自然栖息地，如土壤、空气、发酵食品和人类胃部，都是不同菌株的家园。一些菌株具有独特的优势，由于其多样化的栖息地和生理活性代谢物，在其他微生物群落中广为人知。本研究从哈萨克斯坦阿拉木图的当地市场采集了蔬菜产品（土豆、胡萝卜和番茄）。通过培养后进行生化和系统发育分析来鉴定细菌分离株。我们的系统发育分析显示，三个革兰氏阳性细菌分离株 BSS11、BSS17 和 BSS19 与 O-3、Md1-42 和 Khozestan2 具有 99%的核苷酸序列相似性。从细菌分离株中制备粗提取物，以评估其对抗生素的耐药能力以及针对各种靶向多药耐药菌株的抗菌潜力，包括 、 、 、 、 、 、 、 、 、 、 、 和 。本研究发现，所鉴定的物种具有产生抗生素化学物质的能力。此外，对三个细菌提取物的 GC-MS 分析表明存在许多抗生素物质，包括苯酚、苯甲酸、1,2-苯二甲酸和双（2-甲基丙基）、甲氧苯基肟和苯甲醛。这项工作揭示了 作为一种抗菌剂来靶向不同多药耐药细菌菌株的潜力。结果表明，市场蔬菜可能是一种有用的菌株来源，这些菌株具有一系列有益的特性，可用于生物抗生素的开发。
