Frachini Emilli C G, Selva Jéssica S G, Falcoswki Paula C, Silva Jean B, Cornejo Daniel R, Bertotti Mauro, Ulrich Henning, Petri Denise F S
Departament of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, São Paulo 05508-000, Brazil.
Departament of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo 05508-000, Brazil.
Polymers (Basel). 2023 Mar 31;15(7):1757. doi: 10.3390/polym15071757.
Caffeine (CAF) is a psychostimulant present in many beverages and with rapid bioabsorption. For this reason, matrices that effectuate the sustained release of a low amount of CAF would help reduce the intake frequency and side effects caused by high doses of this stimulant. Thus, in this study, CAF was loaded into magnetic gelatin/alginate (Gel/Alg/MNP) hydrogels at 18.5 mg/g. The in vitro release of CAF was evaluated in the absence and presence of an external magnetic field (EMF) and Ca. In all cases, the presence of Ca (0.002 M) retarded the release of CAF due to favorable interactions between them. Remarkably, the release of CAF from Gel/Alg/MNP in PBS/CaCl (0.002 M) at 37 °C under an EMF was more sustained due to synergic effects. In PBS/CaCl (0.002 M) and at 37 °C, the amounts of CAF released after 45 min from Gel/Alg and Gel/Alg/MNP/EMF were 8.3 ± 0.2 mg/g and 6.1 ± 0.8 mg/g, respectively. The concentration of CAF released from Gel/Alg and Gel/Alg/MNP hydrogels amounted to ~0.35 mM, thereby promoting an increase in cell viability for 48 h. Gel/Alg and Gel/Alg/MNP hydrogels can be applied as reservoirs to release CAF at suitable concentrations, thus forestalling possible side effects and improving the viability of SH-SY5Y cells.
咖啡因（CAF）是一种存在于许多饮料中的精神兴奋剂，具有快速的生物吸收性。因此，能够实现低剂量CAF持续释放的基质将有助于减少这种兴奋剂高剂量摄入所导致的摄入频率和副作用。因此，在本研究中，将CAF以18.5毫克/克的量载入磁性明胶/海藻酸盐（Gel/Alg/MNP）水凝胶中。在不存在和存在外部磁场（EMF）及钙的情况下评估了CAF的体外释放。在所有情况下，由于它们之间的良好相互作用，钙（0.002 M）的存在延缓了CAF的释放。值得注意的是，由于协同效应，在37℃的EMF下，CAF从Gel/Alg/MNP在PBS/CaCl（0.002 M）中的释放更具持续性。在PBS/CaCl（0.002 M）和37℃下，45分钟后从Gel/Alg和Gel/Alg/MNP/EMF释放的CAF量分别为8.3±0.2毫克/克和6.1±0.8毫克/克。从Gel/Alg和Gel/Alg/MNP水凝胶释放的CAF浓度达到约0.35 mM，从而促进细胞活力在48小时内增加。Gel/Alg和Gel/Alg/MNP水凝胶可作为储库以合适的浓度释放CAF，从而预防可能的副作用并提高SH-SY5Y细胞的活力。
