Larijani Milad, Zareshahrabadi Zahra, Alhavaz AbdolHamid, Hajipour Romina, Ranjbaran Alireza, Giti Rashin, Soltankarimi Vahid, Zomorodian Kamiar
Dental Materials Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Curr Med Mycol. 2022 Sep;8(3):23-29. doi: 10.18502/cmm.8.3.11208.
The human mouth mucosal surface is colonized by indigenous microflora, which normally maintains an ecological balance among different species. However, certain environmental or biological factors may disrupt this balance, leading to microbial diseases. biofilms are formed on indwelling medical devices and have an association with both oral and invasive candidiasis. This study aimed to compare the amount of adherent and the biofilm formed on different denture base materials. The adhesion of to denture base materials is widely recognized as the main reason for the development of denture stomatitis.
In total, 56 polymethyl methacrylate (PMMA) acrylic resin disc-shaped samples were divided into four groups as follows: 1) chemically polymerized PMMA, 2) heat-polymerized PMMA, 3) computer-aided design and computer-aided manufacturing (CAD/CAM) PMMA in high polish, and 4) CAD/CAM resins in glazed form. The adherent cells and formation of strains (562, 1905, 1912, and 1949) biofilm were measured by the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) method and use of a microplate reader. Moreover, morphological alterations of cells were investigated using scanning electron microscopy (SEM).
The biofilm formation was significantly lower on CAD/CAM acrylic resins, compared to conventional denture base materials. The obtained results were confirmed by the SEM images of C. albicans biofilms. CAD/CAM PMMA-based polymers may be preferable to inhibit C. albicans biofilm formation and reduce Candida-associated denture stomatitis in long-term use.
Based on the findings, the CAD/CAM technique can be used as an efficient technique for denture fabrication as it inhibits microbial accumulation, and consequently, microbial biofilm.
人类口腔黏膜表面定植着原生微生物群，其通常维持着不同物种间的生态平衡。然而，某些环境或生物因素可能会破坏这种平衡，导致微生物疾病。生物膜在留置医疗器械上形成，并与口腔念珠菌病和侵袭性念珠菌病均有关联。本研究旨在比较不同义齿基托材料上白色念珠菌的黏附量及形成的生物膜。白色念珠菌对义齿基托材料的黏附被广泛认为是义齿性口炎发生的主要原因。
总共56个聚甲基丙烯酸甲酯（PMMA）丙烯酸树脂圆盘形样本被分为以下四组：1）化学聚合PMMA，2）热聚合PMMA，3）高抛光的计算机辅助设计与计算机辅助制造（CAD/CAM）PMMA，4）上釉形式的CAD/CAM树脂。通过2,3-双-(2-甲氧基-4-硝基-5-磺基苯基)-2H-四唑-5-甲酰苯胺（XTT）法并使用酶标仪来测量白色念珠菌菌株（562、1905、1912和1949）生物膜的黏附细胞及形成情况。此外，使用扫描电子显微镜（SEM）研究白色念珠菌细胞的形态改变。
与传统义齿基托材料相比，CAD/CAM丙烯酸树脂上的生物膜形成明显更低。白色念珠菌生物膜的SEM图像证实了所得结果。基于CAD/CAM PMMA的聚合物在长期使用中可能更有利于抑制白色念珠菌生物膜形成并减少念珠菌相关的义齿性口炎。
基于这些发现，CAD/CAM技术可作为一种有效的义齿制作技术，因为它能抑制微生物积聚，进而抑制微生物生物膜形成。
