热循环后不同制造技术制备的各种牙科聚合物的表面性能和微生物黏附的体外研究。
In vitro study of surface properties and microbial adhesion of various dental polymers fabricated by different manufacturing techniques after thermocycling.
机构信息
Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, 88 Jiaotong Road, Fuzhou, 350004, Fujian, China.
Institute of Stomatology & Research Center of Dental Esthetics and Biomechanics, School and Hospital of Stomatology, Fujian Medical University, 246 Yangqiao Zhong Road, Fuzhou, 350002, Fujian, China.
出版信息
Clin Oral Investig. 2022 Dec;26(12):7287-7297. doi: 10.1007/s00784-022-04689-2. Epub 2022 Aug 17.
OBJECTIVES
The study aims to investigate surface properties and microbial adhesion of various dental polymers fabricated by different manufacturing techniques before and after thermocycling.
MATERIALS AND METHODS
The following six materials were used to fabricate disk-shaped specimens: conventional denture polymer (Vertex Acrylic Resin, VAR), CAD/CAM denture polymer (Organic PMMA eco Pink, OP), conventional temporary polymer (Protemp™ 4, PT), CAD/CAM temporary polymer (Die Material, DM), conventional denture framework polymer (BioHPP, PB), and CAD/CAM denture framework polymer (breCAM.BioHPP, CB). The specimens were tested before and after thermocycling (5000 and 10,000 cycles, 5 °C/55 °C). Surface roughness (SR), hydrophobicity, and surface topography were determined by profilometry, water contact angle, and scanning electron microscopy (SEM). Then specimens were incubated with Staphylococcus aureus, Streptococcus mutans, and Candida albicans for 24 h, respectively. Microbial adhesion was assessed using colony-forming unit counts, XTT assay, and SEM.
RESULTS
SR and hydrophobicity of VAR group were higher than that of OP group. S. aureus and C. albicans adhesion on VAR and PT groups were higher than that on OP and DM groups, respectively. There was no difference in surface properties and microbial adhesion between PB and CB groups. After thermocycling, SR (expect OP group) of all materials increased and hydrophobicity decreased, and the amount and activity of S. aureus and C. albicans adhesion also increased. The adhesion of S. aureus and C. albicans showed a moderate positive correlation with SR, independent of hydrophobicity.
CONCLUSIONS
CAD/CAM denture polymers and temporary polymers showed less S. aureus and C. albicans adhesion when compared to conventional ones, which were mainly affected by surface roughness, independent of hydrophobicity. Thermocycling could increase surface roughness, decrease hydrophobicity, and affect microbial adhesion of the materials.
CLINICAL SIGNIFICANCE
CAD/CAM dental polymers may be a better choice for the manufacture of temporary restorations and dentures to reduce microbial adhesion.
目的
本研究旨在探讨不同制造技术制备的各种牙科聚合物在热循环前后的表面性能和微生物黏附。
材料和方法
使用以下六种材料制作圆盘状试件：常规义齿聚合物（Vertex Acrylic Resin，VAR）、CAD/CAM 义齿聚合物（Organic PMMA eco Pink，OP）、常规临时聚合物（Protemp™ 4，PT）、CAD/CAM 临时聚合物（Die Material，DM）、常规义齿框架聚合物（BioHPP，PB）和 CAD/CAM 义齿框架聚合物（breCAM.BioHPP，CB）。在热循环（5000 和 10000 次循环，5°C/55°C）前后测试试件。通过轮廓仪、水接触角和扫描电子显微镜（SEM）测定表面粗糙度（SR）、疏水性和表面形貌。然后，将试件分别与金黄色葡萄球菌、变形链球菌和白色念珠菌孵育 24 小时。使用菌落形成单位计数、XTT 测定和 SEM 评估微生物黏附。
结果
VAR 组的 SR 和疏水性均高于 OP 组。VAR 和 PT 组的金黄色葡萄球菌和白色念珠菌黏附量高于 OP 和 DM 组。PB 和 CB 组的表面性能和微生物黏附无差异。热循环后，所有材料的 SR（OP 组除外）均增加，疏水性降低，金黄色葡萄球菌和白色念珠菌黏附量和活性也增加。金黄色葡萄球菌和白色念珠菌的黏附与 SR 呈中度正相关，与疏水性无关。
结论
与常规聚合物相比，CAD/CAM 义齿聚合物和临时聚合物的金黄色葡萄球菌和白色念珠菌黏附量较少，主要受表面粗糙度影响，与疏水性无关。热循环会增加表面粗糙度，降低疏水性，并影响材料的微生物黏附。
临床意义
CAD/CAM 牙科聚合物可能是制造临时修复体和义齿的更好选择，以减少微生物黏附。
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