Ansarifard Elham, Zareshahrabadi Zahra, Sarafraz Najmeh, Zomorodian Kamiar
Department of Prosthodontics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Nanobiology and Nanomedicine Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Bioinorg Chem Appl. 2021 Jun 4;2021:9939275. doi: 10.1155/2021/9939275. eCollection 2021.
Soft denture liners provide a favorable environment for adhesion and colonization of microorganisms. This in vitro study aimed to examine the efficacy of different concentrations of copper oxide nanoparticles (CuO NPs) incorporation into soft denture liner on the biofilm formation of the microbial species.
Field Emission Scanning Electron Microscopy (FESEM) images from NPs were recorded. Antifungal susceptibility testing of CuO NPs against five standard strains of (CBS 10261, 1905, 1912, 1949, 2730), (ATCC35668), (ATCC27607), and ATCC9222) was performed by the broth microdilution method with the Clinical and Laboratory Standards Institute reference method. The biofilm inhibition percentages of CuO NPs on the soft denture liners were determined by XTT assay.
The characterization of CuO NPs by scanning electron microscope (SEM) analyses confirmed the synthesis of NPs with appropriate structure and size with a mean diameter of 18.3 ± 9.1 nm. The CuO NPs successfully inhibited the growth of the tested standard strains of nd spp. at concentrations ranging from 64 to 128 g mL. Indeed, incorporation of CuO NPs at a concentration of 500 g mL into the soft denture liners exhibited a significant activity (75%) in inhibition of . biofilm formation in a dose-dependent manner. The biofilm formation of in the presence of CuO NPs was lower than spp. in comparison with the control group ( < 0.05).
Incorporation of CuO NPs significantly decreased the colonization and plaque formation of the oral pathogens, especially accumulation. These NPs may be useful as a promising agent for the antimicrobial management of soft denture liner materials.
软质义齿衬垫为微生物的黏附和定植提供了有利环境。本体外研究旨在考察不同浓度的氧化铜纳米颗粒（CuO NPs）掺入软质义齿衬垫对微生物生物膜形成的影响。
记录纳米颗粒的场发射扫描电子显微镜（FESEM）图像。采用肉汤微量稀释法并参照临床和实验室标准协会的方法，对CuO NPs针对五种标准菌株（白假丝酵母菌CBS 10261、1905、1912、1949、2730，光滑假丝酵母菌ATCC35668，热带假丝酵母菌ATCC27607，近平滑假丝酵母菌ATCC9222）进行抗真菌药敏试验。通过XTT法测定CuO NPs对软质义齿衬垫生物膜的抑制率。
通过扫描电子显微镜（SEM）分析对CuO NPs进行表征，证实合成的纳米颗粒具有合适的结构和尺寸，平均直径为18.3±9.1 nm。CuO NPs在浓度为64至128 μg/mL范围内成功抑制了受试的白假丝酵母菌和光滑假丝酵母菌标准菌株的生长。实际上，将浓度为500 μg/mL的CuO NPs掺入软质义齿衬垫中，对光滑假丝酵母菌生物膜形成表现出显著的抑制活性（75%），且呈剂量依赖性。与对照组相比，在CuO NPs存在下光滑假丝酵母菌的生物膜形成低于白假丝酵母菌（P<0.05）。
掺入CuO NPs可显著减少口腔病原体的定植和菌斑形成，尤其是光滑假丝酵母菌的积聚。这些纳米颗粒可能作为软质义齿衬垫材料抗菌处理的一种有前景的制剂。
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