Martinez Peggy, Serpe Marcelo, Barron Rachael, Buerki Sven
Department of Biological Sciences Boise State University Boise Idaho USA.
Department of Plant Sciences Simplot Boise Idaho USA.
Appl Plant Sci. 2023 Mar 2;11(2):e11515. doi: 10.1002/aps3.11515. eCollection 2023 Mar-Apr.
Determining the tolerance of plant populations to climate change requires the development of biotechnological protocols producing genetically identical individuals used for genotype-by-environment experiments. Such protocols are missing for slow-growth, woody plants; to address this gap, this study uses , a western North American keystone shrub, as model.
The production of individual lines is a two-step process: in vitro propagation under aseptic conditions followed by ex vitro acclimation and hardening. Due to aseptic growth conditions, in vitro plantlets exhibit maladapted phenotypes, and this protocol focuses on presenting an approach promoting morphogenesis for slow-growth, woody species. Survival was used as the main criterion determining successful acclimation and hardening. Phenotypic changes were confirmed by inspecting leaf anatomy, and shoot water potential was used to ensure that plantlets were not water stressed.
Although our protocol has lower survival rates (11-41%) compared to protocols developed for herbaceous, fast-growing species, it provides a benchmark for slow-growth, woody species occurring in dry ecosystems.
确定植物种群对气候变化的耐受性需要开发生物技术方案，以产生用于基因型-环境实验的基因相同的个体。对于生长缓慢的木本植物，此类方案尚不存在；为填补这一空白，本研究以北美西部的一种关键灌木——[此处原文缺失具体植物名称]——作为模型。
个体株系的产生是一个两步过程：在无菌条件下进行离体繁殖，然后进行炼苗和驯化。由于无菌生长条件，离体小植株表现出适应不良的表型，本方案重点介绍一种促进生长缓慢的木本物种形态发生的方法。存活率被用作确定成功驯化和炼苗的主要标准。通过检查叶片解剖结构确认表型变化，并利用嫩梢水势确保小植株不受水分胁迫。
尽管与为草本、快速生长物种开发的方案相比，我们的方案存活率较低（11%-41%），但它为干旱生态系统中生长缓慢的木本物种提供了一个基准。
