Okamura Yasushi, Yoshioka Daisuke
Laboratory of Integrative Physiology, Department of Physiology, Graduate School of Medicine, Osaka University, Yamada Oka 2-2, Suita, Osaka 565-0871, Japan.
Biochem Soc Trans. 2023 Apr 26;51(2):827-839. doi: 10.1042/BST20221065.
Many membrane proteins including ion channels and ion transporters are regulated by membrane phospholipids such as phosphoinositides in cell membranes and organelles. Voltage-sensing phosphatase, VSP, is a voltage-sensitive phosphoinositide phosphatase which dephosphorylates PI(4,5)P2 into PI(4)P. VSP rapidly reduces the level of PI(4,5)P2 upon membrane depolarization, thus serving as a useful tool to quantitatively study phosphoinositide-regulation of ion channels and ion transporters using a cellular electrophysiology system. In this review, we focus on the application of VSPs to Kv7 family potassium channels, which have been important research targets in biophysics, pharmacology and medicine.
许多膜蛋白，包括离子通道和离子转运体，都受到细胞膜和细胞器中的膜磷脂（如磷酸肌醇）的调节。电压感应磷酸酶（VSP）是一种电压敏感的磷酸肌醇磷酸酶，它将PI(4,5)P2去磷酸化为PI(4)P。膜去极化时，VSP会迅速降低PI(4,5)P2的水平，因此它是利用细胞电生理系统定量研究磷酸肌醇对离子通道和离子转运体调节作用的有用工具。在这篇综述中，我们重点关注VSP在Kv7家族钾通道中的应用，Kv7家族钾通道一直是生物物理学、药理学和医学领域的重要研究靶点。
