Molecular Oncology Research Center, Barretos Cancer Hospital, Teaching and Research Institute, São Paulo 14784‑400, Brazil.
Department of Gynecological Oncology, Barretos Cancer Hospital, Barretos, São Paulo 14784‑400, Brazil.
Oncol Rep. 2023 May;49(5). doi: 10.3892/or.2023.8546. Epub 2023 Apr 13.
MicroRNAs (miRNAs or miRs) play essential roles in the initiation and progression of human tumors, including cervical cancer. However, the mechanisms underlying their actions in cervical cancer remain unclear. The present study aimed to evaluate the functional role of miR‑130a‑3p in cervical cancer. Cervical cancer cells were transfected with a miRNA inhibitor (anti‑miR‑130a‑3p) and a negative control. Adhesion‑independent cell proliferation, migration and invasion were evaluated. The findings presented herein demonstrated that miR‑130a‑3p was overexpressed in HeLa, SiHa, CaSki, C‑4I and HCB‑514 cervical cancer cells. The inhibition of miR‑130a‑3p significantly reduced the proliferation, migration and invasion of cervical cancer cells. The canonical delta‑like Notch1 ligand () was identified as a possible direct target of miR‑103a‑3p. The gene was further found to be significantly downregulated in cervical cancer tissues. On the whole, the present study demonstrates that miR‑130a‑3p contributes to the proliferation, migration and invasion of cervical cancer cells. Therefore, miR‑130a‑3p may be used as a biomarker to determine cervical cancer progression.
微小 RNA（miRNAs 或 miRs）在人类肿瘤的发生和发展中发挥着重要作用，包括宫颈癌。然而，它们在宫颈癌中的作用机制尚不清楚。本研究旨在评估 miR-130a-3p 在宫颈癌中的功能作用。用 miRNA 抑制剂（anti-miR-130a-3p）和阴性对照转染宫颈癌细胞。评估细胞黏附非依赖性增殖、迁移和侵袭。本文研究结果表明，miR-130a-3p 在 HeLa、SiHa、CaSki、C-4I 和 HCB-514 宫颈癌细胞中过表达。miR-130a-3p 的抑制显著降低了宫颈癌细胞的增殖、迁移和侵袭。经典的 delta-样 Notch1 配体（）被鉴定为 miR-103a-3p 的一个可能的直接靶标。进一步发现基因在宫颈癌组织中显著下调。总的来说，本研究表明 miR-130a-3p 促进了宫颈癌细胞的增殖、迁移和侵袭。因此，miR-130a-3p 可作为判断宫颈癌进展的生物标志物。
