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NR5A2 作为运动改善代谢综合征的潜在靶点。

NR5A2 as a potential target for exercise to improve metabolic syndrome.

机构信息

Department of Cardiology, Qinhuangdao Second Hospital, Qinhuangdao, Hebei 066600, PR China.

Department of Anesthesiology, Qinhuangdao Second Hospital, Qinhuangdao, Hebei 066600, PR China.

出版信息

Aging (Albany NY). 2023 Mar 26;15(7):2485-2502. doi: 10.18632/aging.204606.

DOI:10.18632/aging.204606
PMID:37053002
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10120892/
Abstract

BACKGROUND

Metabolic syndrome is a syndrome of a variety of metabolic disorders. Exercise is beneficial to the human body. However, the association of NR5A2 and exercise with metabolic syndrome remains unclear.

METHODS

Download the GSE10540 and GSE12385 from GEO database. Bioinformatics analysis was used to screen the hub molecular of the metabolic syndrome. Forty 3-week-old C57BL/6J male mice were used in this study. The mean body weight was (17.5 ± 2.1) g. After 10 days of adaptive feeding, they were randomly divided into 4 groups according to the random number table method: Model + Exercise ( = 10), Model ( = 10), Model/NR5A2-OE ( = 10), Model/NR5A2-KO ( = 10). Western Blotting was performed to detect the expression of hub genes and signaling pathway.

RESULTS

There were 349 DEGs in GSE10540 and 49 DEGs in GSE12385. 10 core genes were obtained. GO showed that differentially expressed genes were mainly enriched in vascular morphogenesis, contractile fiber fraction, chemotaxis, and MAPK cascade regulation. KEGG showed that MAPK signaling pathway was a significant section in the metabolic syndrome. PIK3R2, STRA8, FLT1, DMRT1, FGF22, NR5A2, and FLT were up-regulated and PRDM14, POU5F1, and KDR were down-regulated in metabolic syndrome after exercise.

CONCLUSION

The expression of NR5A2 is down-regulated in metabolic syndrome, and exercise can increase the expression level of NR5A2. NR5A2 might be used as a potential target for exercise to improve metabolic syndrome.

摘要

背景

代谢综合征是多种代谢紊乱的综合征。运动对人体有益。然而，NR5A2 与运动和代谢综合征的关系尚不清楚。

方法

从 GEO 数据库中下载 GSE10540 和 GSE12385。使用生物信息学分析筛选代谢综合征的枢纽分子。本研究使用 40 只 3 周龄 C57BL/6J 雄性小鼠。平均体重为（17.5±2.1）g。适应性喂养 10 天后，根据随机数字表法将其随机分为 4 组：模型+运动组（n=10）、模型组（n=10）、模型/NR5A2-OE 组（n=10）、模型/NR5A2-KO 组（n=10）。Western Blotting 检测枢纽基因和信号通路的表达。

结果

GSE10540 中有 349 个 DEGs，GSE12385 中有 49 个 DEGs。获得 10 个核心基因。GO 显示差异表达基因主要富集在血管形态发生、收缩纤维分数、趋化作用和 MAPK 级联调节。KEGG 显示 MAPK 信号通路是代谢综合征的一个显著部分。运动后代谢综合征中 PIK3R2、STRA8、FLT1、DMRT1、FGF22、NR5A2 和 FLT 上调，PRDM14、POU5F1 和 KDR 下调。

结论

NR5A2 在代谢综合征中表达下调，运动可以增加 NR5A2 的表达水平。NR5A2 可能成为运动改善代谢综合征的潜在靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d8/10120892/dbdcb05da6e2/aging-15-204606-g012.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d8/10120892/95d3bccf0a0e/aging-15-204606-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d8/10120892/ae7b48e4cc53/aging-15-204606-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d8/10120892/dbdcb05da6e2/aging-15-204606-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d8/10120892/ce5bd824e36d/aging-15-204606-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d8/10120892/9e3dca36e4b4/aging-15-204606-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d8/10120892/6e2b8a55a93f/aging-15-204606-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d8/10120892/efddfeb49c61/aging-15-204606-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d8/10120892/3255df9e73e1/aging-15-204606-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d8/10120892/95d3bccf0a0e/aging-15-204606-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d8/10120892/ae7b48e4cc53/aging-15-204606-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d8/10120892/197b7712b1c7/aging-15-204606-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d8/10120892/b197e14eafb0/aging-15-204606-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d8/10120892/7fb5e8032571/aging-15-204606-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d8/10120892/dbdcb05da6e2/aging-15-204606-g012.jpg

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