State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Sep 5;297:122673. doi: 10.1016/j.saa.2023.122673. Epub 2023 Mar 31.
As glyphosate is a broad-spectrum herbicide extensively used in agriculture worldwide, rapid glyphosate detection is essential for food safety and human health. Herein, a ratio fluorescence test strip was prepared and coupled with an amino-functionalized bismuth-based metal-organic framework (NH-Bi-MOF) that bonded with copper ion for rapid visualization and determination of glyphosate. NH-Bi-MOF had excellent fluorescence performance, and the copper ion, a Lewis acid, was selected as the quencher. The strong chelation of glyphosate with copper ion and its quick interaction with NH-Bi-MOF would turn on the fluorescence signal, thus enabling the quantitative sensing of glyphosate, with a linear range of 0.10-200 µmol L, and recoveries between 94.8% and 113.5%. The system was then expanded to a ratio fluorescence test strip, in which the fluorescent ring sticker was set as a binding-in self-calibration to reduce errors from the angle and light dependency. The method realized the visual semi-quantitation referring to a standard card, as well as the ratio quantitation using the gray value output with LOD of 0.82 µmol L. And the as-developed test strip was accessible, portable, and reliable, thus offering a platform for the rapid on-site detection of glyphosate and other residual pesticides.
由于草甘膦是一种在全世界范围内广泛用于农业的广谱除草剂，因此快速检测草甘膦对于食品安全和人类健康至关重要。本文制备了比率荧光测试条，并将其与氨基功能化的铋基金属有机骨架（NH-Bi-MOF）偶联，该 MOF 键合有铜离子，可用于快速可视化和测定草甘膦。NH-Bi-MOF 具有出色的荧光性能，而铜离子作为路易斯酸被选为猝灭剂。草甘膦与铜离子的强烈螯合作用及其与 NH-Bi-MOF 的快速相互作用会开启荧光信号，从而实现对草甘膦的定量传感，线性范围为 0.10-200 μmol L，回收率在 94.8%-113.5%之间。然后，该系统扩展到比率荧光测试条，其中荧光环贴纸被设置为绑定自校准，以减少角度和光依赖性引起的误差。该方法通过参考标准卡实现了目视半定量，以及通过使用灰度值输出进行比率定量（LOD 为 0.82 μmol L）。所开发的测试条易于使用、便携且可靠，因此为快速现场检测草甘膦和其他残留农药提供了一个平台。
