Medical School of Chinese PLA, Beijing, 100853, China.
Department of Nephrology, First Medical Center of Chinese PLA General Hospital, Nephrology Institute of the Chinese People's Liberation Army, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Disease Research, Beijing, 100853, China.
Sci Rep. 2023 Apr 13;13(1):6048. doi: 10.1038/s41598-023-32910-z.
The most prevalent primary glomerulonephritis and leading cause of end-stage renal disease worldwide is IgA nephropathy (IgAN). More and more studies are describing urinary microRNA (miRNA) as a non-invasive marker for a variety of renal diseases. We screened candidate miRNAs based on data from three published IgAN urinary sediment miRNAs chips. In separate confirmation and validation cohorts, we included 174 IgAN patients, 100 patients with other nephropathies as disease controls (DC), and 97 normal controls (NC) for quantitative real-time PCR. A total of three candidate miRNAs, miR-16-5p, Let-7g-5p, miR-15a-5p were obtained. In both the confirmation and validation cohorts, these miRNAs levels were considerably higher in the IgAN than in NC, with miR-16-5p significantly higher than in DC. The area under the ROC curve for urinary miR-16-5p levels was 0.73. Correlation analysis suggested that miR-16-5p was positively correlated with endocapillary hypercellularity (r = 0.164 p = 0.031). When miR-16-5p was combined with eGFR, proteinuria and C4, the AUC value for predicting endocapillary hypercellularity was 0.726. By following the renal function of patients with IgAN, the levels of miR-16-5p were noticeably higher in the IgAN progressors than in the non- progressors (p = 0.036). Urinary sediment miR-16-5p can be used as noninvasive biomarkers for the assessment of endocapillary hypercellularity and diagnosis of IgA nephropathy. Furthermore, urinary miR-16-5p may be predictors of renal progression.
最常见的原发性肾小球肾炎和全球范围内导致终末期肾病的主要原因是 IgA 肾病 (IgAN)。越来越多的研究将尿 microRNA (miRNA) 描述为各种肾脏疾病的非侵入性标志物。我们根据三篇已发表的 IgAN 尿沉渣 miRNA 芯片数据筛选候选 miRNA。在单独的确认和验证队列中，我们纳入了 174 例 IgAN 患者、100 例其他肾病患者作为疾病对照 (DC) 和 97 例正常对照 (NC) 进行实时定量 PCR。总共获得了三个候选 miRNA，miR-16-5p、Let-7g-5p 和 miR-15a-5p。在确认和验证队列中，这些 miRNA 在 IgAN 患者中的水平明显高于 NC，miR-16-5p 明显高于 DC。尿 miR-16-5p 水平的 ROC 曲线下面积为 0.73。相关性分析表明 miR-16-5p 与毛细血管内细胞增多呈正相关 (r = 0.164，p = 0.031)。当 miR-16-5p 与 eGFR、蛋白尿和 C4 联合使用时，预测毛细血管内细胞增多的 AUC 值为 0.726。通过随访 IgAN 患者的肾功能，进展组的 miR-16-5p 水平明显高于非进展组 (p = 0.036)。尿沉渣 miR-16-5p 可作为评估毛细血管内细胞增多和诊断 IgA 肾病的非侵入性生物标志物。此外，尿 miR-16-5p 可能是肾脏进展的预测因子。
