Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Science, China West Normal University, Nanchong, 637009, Sichuan, China.
College of Environmental Science and Engineering, China West Normal University, Nanchong, 637009, Sichuan, China.
Funct Integr Genomics. 2023 Apr 13;23(2):123. doi: 10.1007/s10142-023-01052-w.
Multi-pistil trait in wheat is of great potential value in plant development research and crop breeding. Our previous studies identified the Pis1 locus that causes three pistils in wheat by genetic mapping using multiple DNA marker systems. However, there are still 26 candidate genes on the locus, and the causal gene remains to be found. In this study, we aimed to approach the molecular mechanism of multi-pistil formation. Comparative RNA sequencing (RNA-Seq) during the pistil formation was undertaken in four wheat lines: a three-pistil mutant TP, a single-pistil TILLING mutant of TP (SP), a three-pistil near-isogenic line CM28TP with the background of cultivar Chunmai 28 (CM28), and CM28. Electron microscopic analysis specified probable developmental stages of young spikes for the three-pistil formation. mRNA sequencing in the young spikes of the four lines represented 253 down-regulated genes and 98 up-regulated genes in both three-pistil lines, which included six potential genes for ovary development. Weighted gene co-expression analysis represented three-pistil trait-associated transcription factor-like genes, among which one hub gene, ARF5, was the most highlighted. ARF5 is on the Pis1 locus and an orthologue of MONOPTEROS which mediates tissue development in Arabidopsis. qRT-PCR validation implies that the deficiency of ARF5 underlies the three-pistil formation in wheat.
小麦多雌蕊性状在植物发育研究和作物育种中具有巨大的潜在价值。我们之前的研究通过使用多个 DNA 标记系统的遗传图谱，鉴定了导致小麦三雌蕊的 Pis1 基因座。然而，该基因座上仍有 26 个候选基因，其因果基因仍有待发现。在这项研究中，我们旨在研究多雌蕊形成的分子机制。在四个小麦品系中进行了雌蕊形成过程中的比较 RNA 测序（RNA-Seq）：一个三雌蕊突变体 TP、TP 的单雌蕊 TILLING 突变体（SP）、具有春麦 28 品种背景的三雌蕊近等基因系 CM28TP（CM28）和 CM28。电子显微镜分析指定了三个雌蕊形成的幼穗的可能发育阶段。四个品系的幼穗 mRNA 测序显示，两个三雌蕊品系中有 253 个下调基因和 98 个上调基因，其中包括 6 个潜在的卵巢发育相关基因。加权基因共表达分析代表了三雌蕊性状相关的转录因子样基因，其中一个枢纽基因 ARF5 最为突出。ARF5 位于 Pis1 基因座上，是拟南芥中介导组织发育的 MONOPTEROS 的同源基因。qRT-PCR 验证表明，ARF5 的缺失是小麦三雌蕊形成的基础。
