Li Xiang, Li Zitao, Li Caijuan, Wang Xuemei, Jiang Bin, Wang Jiannan, Zhang Zhen
Department of Ultrasonic Diagnosis, The First Hospital of China Medical University, 155 Nanjing North Street, Shenyang 110001, Liaoning, PR China.
Department of Orthopedic Surgery, Hongqi Hospital, Mudanjiang Medical University, 5 Tongxiang Road, Mudanjiang, Heilongjiang 157011, PR China.
J Cancer. 2023 Mar 5;14(4):665-675. doi: 10.7150/jca.82438. eCollection 2023.
The purpose of this study was to clarify the effect of C-X-C chemokine receptor type 7 (CXCR7) on proliferation, migration, and angiogenesis by changing the expression levels of CXCR7 in colon cancer cells. Contrast-enhanced ultrasound technology was used to quantify tumor perfusion parameters for the detection of angiogenesis after the change of CXCR7 expression in colon cancer xenografts. To detect the expression of CXCR7 in colon cancer cells after overexpression or silencing of CXCR7. In addition, proliferation, migration, and angiogenesis were determined. The region of interest of the tumor was selected, and a time-intensity curve was drawn. Immunohistochemical staining was performed on tumor tissue sections, and the average microvessel density value was calculated. Overexpression or silencing of CXCR7 altered the proliferation, migration, and luminal formation of Caco-2 and SW480 cells. In xenografts produced using CXCR7-overexpressing or -silent Caco-2 and SW480, respectively, the peak intensity and area under the curve were significantly different. The expression of CXCR7, VEGF, Ki67, and CD34 was decreased in CXCR7-silent cells, but increased in CXCR7-overexpressing cells. CXCR7 apparently affected angiogenesis through the extracellular signal regulated kinase pathway. The regulation of CXCR7 expression may affect the proliferation, migration, and luminal formation of Caco-2 and SW480 cells, indicating that CXCR7 may play an important role in colon cancer. Examination through contrast-enhanced ultrasound also demonstrated that the expression of CXCR7 is closely related to angiogenesis.
本研究的目的是通过改变结肠癌细胞中C-X-C趋化因子受体7（CXCR7）的表达水平，阐明其对增殖、迁移和血管生成的影响。在结肠癌异种移植瘤中CXCR7表达改变后，采用超声造影技术定量肿瘤灌注参数以检测血管生成。检测CXCR7过表达或沉默后结肠癌细胞中CXCR7的表达。此外，还测定了增殖、迁移和血管生成情况。选择肿瘤的感兴趣区域并绘制时间-强度曲线。对肿瘤组织切片进行免疫组织化学染色，并计算平均微血管密度值。CXCR7的过表达或沉默改变了Caco-2和SW480细胞的增殖、迁移和管腔形成。在分别使用过表达或沉默CXCR7的Caco-2和SW480产生的异种移植瘤中，峰值强度和曲线下面积有显著差异。CXCR7沉默细胞中CXCR7、VEGF、Ki67和CD34的表达降低，但在CXCR7过表达细胞中升高。CXCR7显然通过细胞外信号调节激酶途径影响血管生成。CXCR7表达的调节可能影响Caco-2和SW480细胞的增殖、迁移和管腔形成，表明CXCR7可能在结肠癌中起重要作用。通过超声造影检查还表明，CXCR7的表达与血管生成密切相关。
