School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, China; Shaanxi Engineering Research Center of Cardiovascular Drugs Screening & Analysis, Xi'an, 710061, China.
School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, China; Shaanxi Engineering Research Center of Cardiovascular Drugs Screening & Analysis, Xi'an, 710061, China.
J Chromatogr A. 2021 Aug 30;1652:462349. doi: 10.1016/j.chroma.2021.462349. Epub 2021 Jun 15.
In this study, a zirconium-based metal-organic framework UiO-66-NH modified cotton fiber (CF@UiO-66-NH) was fabricated for the extraction of five common NSAIDs, namely ketoprofen, naproxen, flurbiprofen, diclofenac sodium, and ibuprofen. UiO-66-NH was synthesized and immobilized on the surface of cotton fiber using an environmentally friendly aqueous synthesis method. The prepared CF@UiO-66-NH composite of 50 mg was loaded into a 13 mm recessed filter for use as a solid-phase extraction (SPE) adsorbent material. The filter was then used to enrich NSAIDs in fish and shrimp muscle tissues followed by ultra-high performance liquid chromatography (UPLC) detection. Several key parameters were evaluated and optimized, including adsorption flow rate, pH value of sample, desorption flow rate, and the formic acid content of the eluent. Under optimized conditions, linear ranges of ketoprofen, naproxen, flurbiprofen, diclofenac sodium, and ibuprofen were 2.0-300.0 ng/mL, 1.4-280.0 ng/mL, 3.0-400.0 ng/mL, 1.0-500.0 ng/mL, and 14.0-560.0 ng/mL, respectively. The detection limits ranged from 0.12 ng/mL to 3.50 ng/mL with recoveries in the range of 72.95-116.99%, RSDs < 9.90%. The results demonstrated that the homemade filters based on CF@UiO-66-NH exhibited good reproducibility, stability and adsorption property for the determination of trace-level NSAIDs in complex matrix.
在这项研究中，制备了一种基于锆的金属有机骨架 UiO-66-NH 修饰的棉纤维（CF@UiO-66-NH），用于提取五种常见的非甾体抗炎药，即酮洛芬、萘普生、氟比洛芬、双氯芬酸钠和布洛芬。UiO-66-NH 是通过一种环保的水相合成方法合成并固定在棉纤维表面上的。制备的 50mg CF@UiO-66-NH 复合材料被装入 13mm 凹形过滤器中，用作固相萃取（SPE）吸附剂材料。然后使用该过滤器从鱼类和虾类肌肉组织中富集 NSAIDs，再进行超高效液相色谱（UPLC）检测。评估和优化了几个关键参数，包括吸附流速、样品 pH 值、解吸流速和洗脱剂中甲酸的含量。在优化条件下，酮洛芬、萘普生、氟比洛芬、双氯芬酸钠和布洛芬的线性范围分别为 2.0-300.0ng/mL、1.4-280.0ng/mL、3.0-400.0ng/mL、1.0-500.0ng/mL 和 14.0-560.0ng/mL。检测限范围为 0.12ng/mL 至 3.50ng/mL，回收率在 72.95%-116.99%之间，RSDs<9.90%。结果表明，基于 CF@UiO-66-NH 的自制过滤器对复杂基质中痕量 NSAIDs 的测定具有良好的重现性、稳定性和吸附性能。
