College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao 266003, China.
College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China.
Carbohydr Polym. 2023 Jul 15;312:120817. doi: 10.1016/j.carbpol.2023.120817. Epub 2023 Mar 16.
In the past few decades, sulfated fucan from sea cucumber had attracted considerable interest owing to its abundant physiological activities. Nevertheless, its potential for species discrimination had not been investigated. Herein, particular attention was given to sea cucumber Apostichopus japonicus, Acaudina molpadioides, Holothuria hilla, Holothuria tubulosa, Isostichopus badionotus and Thelenota ananas to examine the feasibility of sulfated fucan as a species marker of sea cucumber. The enzymatic fingerprint suggested that sulfated fucan exhibited significant interspecific discrepancy and intraspecific stability, which revealed that sulfated fucan could serve as the species marker of sea cucumber, by utilizing the overexpressed endo-1,3-fucanase Fun168A and the ultra-performance liquid chromatography-high resolution mass spectrum. Moreover, oligosaccharide profile of sulfated fucan was determined. The oligosaccharide profile combined with hierarchical clustering analysis and principal components analysis further confirmed that sulfated fucan could serve as a marker with a satisfying performance. Besides, load factor analysis showed that the minor structure of sulfated fucan also contributed to the sea cucumber discrimination, besides the major structure. The overexpressed fucanase played an indispensable role in the discrimination, due to its specificity and high activity. The study would lead to a new strategy for species discrimination of sea cucumber based on sulfated fucan.
在过去几十年中，海参中的硫酸化岩藻聚糖因其丰富的生理活性而引起了相当大的关注。然而，其在物种鉴别方面的潜力尚未得到研究。本文特别关注海参糙海参、海地瓜、刺参、海棒槌和图纹白尼参，以检验硫酸化岩藻聚糖作为海参物种标记物的可行性。酶指纹图谱表明，硫酸化岩藻聚糖表现出显著的种间差异和种内稳定性，这表明硫酸化岩藻聚糖可以作为海参的物种标记物，利用过表达的内切-1,3-岩藻聚糖酶 Fun168A 和超高效液相色谱-高分辨质谱。此外，还确定了硫酸化岩藻聚糖的低聚糖图谱。低聚糖图谱结合层次聚类分析和主成分分析进一步证实，硫酸化岩藻聚糖可以作为一种具有令人满意性能的标记物。此外，负荷因子分析表明，除了主要结构外，硫酸化岩藻聚糖的次要结构也有助于海参的鉴别。过表达的岩藻聚糖酶由于其特异性和高活性，在鉴别中起着不可或缺的作用。该研究将为基于硫酸化岩藻聚糖的海参物种鉴别提供一种新策略。
