Hakes D C, Johnson G D, Marhevka J S
Am Ind Hyg Assoc J. 1986 Mar;47(3):181-4. doi: 10.1080/15298668691389559.
The chromatographic problems associated with the excess derivatizing reagent, N-4-nitrobenzyl-N-n-propylamine, in the commonly used HPLC method for the determination of isocyanates have been eliminated. An aqueous extraction removed the excess reagent without affecting the concentration of the urea derivatives. The combination of the extraction with the use of a fast-LC column allowed separation and quantitation of 4,4'-diphenylmethane diisocyanate, 2,4- and 2,6-toluene diisocyanate and hexamethylene diisocyanate within four minutes under isocratic conditions. Detection limits ranged from 2 to 5 ng injected.
在常用的用于测定异氰酸酯的高效液相色谱法中，与过量衍生试剂N-4-硝基苄基-N-正丙胺相关的色谱问题已得到解决。水相萃取可去除过量试剂，而不影响脲衍生物的浓度。萃取与使用快速液相色谱柱相结合，可在等度条件下于四分钟内分离并定量4,4'-二苯基甲烷二异氰酸酯、2,4-和2,6-甲苯二异氰酸酯以及六亚甲基二异氰酸酯。进样检测限为2至5 ng。
