Wu W S, Stoyanoff R E, Szklar R S, Gaind V S, Rakanovic M
Occupational Health Laboratory, Ontario Ministry of Labour, Weston, Canada.
Analyst. 1990 Jun;115(6):801-7. doi: 10.1039/an9901500801.
Determination of total airborne isocyanates using tryptamine as the derivatising agent was investigated. Tryptamine derivatised isocyanates were analysed by reversed-phase high-performance liquid chromatography (HPLC). The column was equipped with dual detectors of fluorescence emission and amperometric oxidation. The characteristics of fluorescence emission and amperometric oxidation of tryptamine were retained even after its reaction with isocyanates. With this unique behaviour, all tryptamine derivatised isocyanates can be quantified using HPLC by employing a single, pure derivative, such as tryptamine derivatised hexamethylene diisocyanate as the calibration standard. This is especially important for analysing polymeric isocyanates when identical calibration standards are not always available. The applicability of this method for air sampling was evaluated by comparison with the established method of Bagon et al. involving 1-(2-methoxyphenyl)piperazine. Simulation of air sampling was performed in a Test Atmosphere Generation System by the vaporisation of toluene diisocyanate. Satisfactory results were obtained, indicating the applicability of this technique for the determination of total airborne isocyanates.
研究了以色胺作为衍生剂测定空气中异氰酸酯总量的方法。色胺衍生的异氰酸酯通过反相高效液相色谱（HPLC）进行分析。该色谱柱配备了荧光发射和安培氧化双检测器。即使色胺与异氰酸酯反应后，其荧光发射和安培氧化特性仍得以保留。凭借这种独特的性质，所有色胺衍生的异氰酸酯都可以通过HPLC进行定量，采用单一的纯衍生物，如色胺衍生的六亚甲基二异氰酸酯作为校准标准品。当并非总能获得相同的校准标准品时，这对于分析聚合异氰酸酯尤为重要。通过与Bagon等人建立的涉及1-（2-甲氧基苯基）哌嗪的方法进行比较，评估了该方法在空气采样中的适用性。通过甲苯二异氰酸酯的汽化，在试验大气生成系统中进行了空气采样模拟。获得了满意的结果，表明该技术可用于测定空气中异氰酸酯总量。
