Pande Pranav M, Azarbad Hamed, Tremblay Julien, St-Arnaud Marc, Yergeau Etienne
Institut national de la recherche scientifique, Centre Armand-Frappier Santé Biotechnologie, Laval, Québec, H7V 1B7, Canada.
Department of Biology, Evolutionary Ecology of Plants, Philipps-University Marburg, Marburg, Germany.
ISME Commun. 2023 Apr 15;3(1):30. doi: 10.1038/s43705-023-00235-7.
Crops associate with microorganisms that help their resistance to biotic stress. However, it is not clear how the different partners of this association react during exposure to stress. This knowledge is needed to target the right partners when trying to adapt crops to climate change. Here, we grew wheat in the field under rainout shelters that let through 100%, 75%, 50% and 25% of the precipitation. At the peak of the growing season, we sampled plant roots and rhizosphere, and extracted and sequenced their RNA. We compared the 100% and the 25% treatments using differential abundance analysis. In the roots, most of the differentially abundant (DA) transcripts belonged to the fungi, and most were more abundant in the 25% precipitation treatment. About 10% of the DA transcripts belonged to the plant and most were less abundant in the 25% precipitation treatment. In the rhizosphere, most of the DA transcripts belonged to the bacteria and were generally more abundant in the 25% precipitation treatment. Taken together, our results show that the transcriptomic response of the wheat holobiont to decreasing precipitation levels is stronger for the fungal and bacterial partners than for the plant.
作物与有助于其抵抗生物胁迫的微生物相关联。然而，尚不清楚这种关联中的不同伙伴在受到胁迫时会如何反应。在试图使作物适应气候变化时，需要这些知识来确定合适的伙伴。在这里，我们在防雨棚下的田间种植小麦，防雨棚分别能让100%、75%、50%和25%的降水通过。在生长季节的高峰期，我们采集了植物根系和根际样本，并提取和测序了它们的RNA。我们使用差异丰度分析比较了100%和25%处理组。在根系中，大多数差异丰度（DA）转录本属于真菌，且大多数在25%降水处理组中更为丰富。约10%的DA转录本属于植物，且大多数在25%降水处理组中丰度较低。在根际中，大多数DA转录本属于细菌，且通常在25%降水处理组中更为丰富。综合来看，我们的结果表明，小麦整体生物对降水水平降低的转录组反应，真菌和细菌伙伴比植物更强。
