使用傅里叶变换红外光谱进行细菌分型的方案。
Protocol for bacterial typing using Fourier transform infrared spectroscopy.
作者信息
Yang Huayan, Shi Haimei, Feng Bin, Wang Li, Chen Liangqiang, Alvarez-Ordóñez Avelino, Zhang Li, Shen Hao, Zhu Jianhua, Yang Shouning, Ding Chuanfan, Prietod Miguel, Yang Fan, Yu Shaoning
机构信息
Department of Intensive Care Unit, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang 315211, China; Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China.
Kweichow Moutai Group, Renhuai, Guizhou 564501, China.
出版信息
STAR Protoc. 2023 Apr 15;4(2):102223. doi: 10.1016/j.xpro.2023.102223.
The Fourier transform infrared (FT-IR) signals obtained from bacterial samples are specific and reproducible, making FT-IR an efficient tool for bacterial typing at a subspecies level. However, the typing accuracy could be affected by many factors, including sample preparation and spectral acquisition. Here, we present a unified protocol for bacterial typing based on FT-IR spectroscopy. We describe sample preparation from bacterial culture and FT-IR spectrum collection. We then detail FT-IR spectrum preprocessing and multivariate analysis of spectral data for bacterial typing.
从细菌样本中获得的傅里叶变换红外（FT-IR）信号具有特异性和可重复性，这使得FT-IR成为亚种水平细菌分型的有效工具。然而，分型准确性可能受到许多因素的影响，包括样本制备和光谱采集。在此，我们提出了一种基于FT-IR光谱的细菌分型统一方案。我们描述了从细菌培养物进行样本制备以及FT-IR光谱采集的过程。然后，我们详细介绍了用于细菌分型的FT-IR光谱预处理和光谱数据的多变量分析。
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