Shaheed Rajguru College of Applied Sciences for Women, University of Delhi, New Delhi, India.
School of Physical Sciences, Jawaharlal Nehru University, New Delhi, India.
Oxid Med Cell Longev. 2023 Apr 6;2023:7707452. doi: 10.1155/2023/7707452. eCollection 2023.
Cadmium selenium quantum dots (CdSe QDs) with modified surfaces exhibit superior dispersion stability and high fluorescence yield, making them desirable biological probes. The knowledge of cellular and biochemical toxicity has been lacking, and there is little information on the correlation between and data. The current study was carried out to assess the toxicity of CdSe QDs after intravenous injection in Wistar male rats (230 g). The rats were given a single dose of QDs of 10, 20, 40, and 80 mg/kg and were kept for 30 days. Following that, various biochemical assays, hematological parameters, and bioaccumulation studies were carried out. Functional as well as clinically significant changes were observed. There was a significant increase in WBC while the RBC decreased. This suggested that CdSe quantum dots had inflammatory effects on the treated rats. The various biochemical assays clearly showed that high dose induced hepatic injury. At a dose of 80 mg/kg, bioaccumulation studies revealed that the spleen (120 g/g), liver (78 g/g), and lungs (38 g/g) accumulated the most. In treated Wistar rats, the bioretention profile of QDs was in the following order: the spleen, liver, kidney, lungs, heart, brain, and testis. The accumulation of these QDs induced the generation of intracellular reactive oxygen species, resulting in an alteration in antioxidant activity. It is concluded that these QDs caused oxidative stress, which harmed cellular functions and, under certain conditions, caused partial brain, kidney, spleen, and liver dysfunction. This is one of the most comprehensive studies on the nanotoxicity of CdSe quantum dots.
镉硒量子点（CdSe QDs）经过表面修饰后，表现出卓越的分散稳定性和高荧光产率，是理想的生物探针。然而，关于其细胞和生化毒性的认识还很缺乏，并且关于与毒性数据的相关性的信息也很少。本研究旨在评估静脉注射 Wistar 雄性大鼠（230g）后 CdSe QDs 的毒性。大鼠单次给予 10、20、40 和 80mg/kg 的 QDs，并饲养 30 天。之后，进行了各种生化分析、血液学参数和生物累积研究。结果观察到了功能和临床相关的变化。白细胞计数显著增加，而红细胞计数减少。这表明 CdSe 量子点对处理后的大鼠具有炎症作用。各种生化分析清楚地表明，高剂量会导致肝损伤。在 80mg/kg 剂量下，生物累积研究表明脾脏（120g/g）、肝脏（78g/g）和肺部（38g/g）积累最多。在处理过的 Wistar 大鼠中，QDs 的生物保留特征如下：脾脏、肝脏、肾脏、肺部、心脏、大脑和睾丸。这些 QDs 的积累诱导了细胞内活性氧的产生，导致抗氧化活性发生改变。结论是，这些 QDs 引起了氧化应激，损害了细胞功能，在某些情况下导致了部分脑、肾、脾和肝功能障碍。这是关于 CdSe 量子点纳米毒性的最全面的研究之一。
