Department of Dairy Science and Food Technology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India.
Faculty of Veterinary and Animal Sciences, Institute of Agricultural Sciences, Banaras Hindu University, Mirzapur, India.
J Sci Food Agric. 2023 Aug 30;103(11):5634-5640. doi: 10.1002/jsfa.12640. Epub 2023 May 1.
Milk contains a massive class of minor proteins that are known for their various biological and molecular functions. Many whey proteins transfer the host defense mechanism to the human body. In this assay, electrophoresis followed by a high-resolution mass spectrometry-based proteomic approach has been applied to identify the whey proteome of Indian Jersey crossbreed bovines.
Two search engines, MS Amanda and Sequest HT, have shown more than 29 minor proteins. Chromosomal mapping revealed that chromosomes 5 and 9 are expressing maximum proteins in the whey proteome. The principal component analysis, outlier plots, scree plots, score plots, and loading plots were generated to further assess the results.
The majorly expressed ones are glycosylation-dependent cell adhesion molecule-1, ubiquitin, desmoglein, annexin, glycoprotein, arginase, histones, peroxiredoxin, vimentin, desmin, catenin, peripherin, and 70 kDa heat shock protein. © 2023 Society of Chemical Industry.
牛奶含有大量的小类蛋白质，这些蛋白质以其各种生物和分子功能而闻名。许多乳清蛋白将宿主防御机制转移到人体中。在这项实验中，应用电泳和基于高分辨率质谱的蛋白质组学方法来鉴定印度泽西杂交奶牛的乳清蛋白质组。
两个搜索引擎，MS Amanda 和 Sequest HT，已经鉴定出超过 29 种小类蛋白质。染色体作图显示，染色体 5 和 9 是乳清蛋白质组中表达最多的蛋白质。还生成了主成分分析、离群值图、 scree 图、得分图和加载图，以进一步评估结果。
主要表达的蛋白质是糖基化依赖的细胞黏附分子-1、泛素、桥粒芯糖蛋白、膜联蛋白、糖蛋白、精氨酸酶、组蛋白、过氧化物酶、波形蛋白、结蛋白、连接蛋白、周围蛋白和 70 kDa 热休克蛋白。© 2023 化学工业协会。
