Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.
Institute of Animal Hygiene and Environmental Health, Centre for Infectious Medicine, Freie Universität Berlin, 14163 Berlin, Germany.
Int J Mol Sci. 2017 Dec 28;19(1):78. doi: 10.3390/ijms19010078.
is the most common contagious pathogen associated with bovine subclinical mastitis. Current diagnosis of mastitis is based on bacteriological culture of milk samples and somatic cell counts, which lack either sensitivity or specificity. Identification of milk proteins that contribute to host defense and their variable responses to pathogenic stimuli would enable the characterization of putative biomarkers of subclinical mastitis. To accomplish this, milk whey samples from healthy and mastitic dairy cows were analyzed using a label-free quantitative proteomics approach. In total, 90 proteins were identified, of which 25 showed significant differential abundance between healthy and mastitic samples. In silico functional analyses indicated the involvement of the differentially abundant proteins in biological mechanisms and signaling pathways related to host defense including pathogen-recognition, direct antimicrobial function, and the acute-phase response. This proteomics and bioinformatics analysis not only facilitates the identification of putative biomarkers of subclinical mastitis but also recapitulates previous findings demonstrating the abundance of host defense proteins in intramammary infection. All mass spectrometry data are available via ProteomeXchange with identifier .
是与牛亚临床乳腺炎相关的最常见的传染性病原体。目前乳腺炎的诊断基于奶样的细菌培养和体细胞计数，但这些方法要么缺乏敏感性，要么缺乏特异性。鉴定有助于宿主防御的乳蛋白及其对致病刺激的可变反应将能够表征亚临床乳腺炎的潜在生物标志物。为了实现这一目标，使用无标记定量蛋白质组学方法分析了健康和乳腺炎奶牛的乳清样本。总共鉴定了 90 种蛋白质，其中 25 种在健康和乳腺炎样本之间表现出显著的差异丰度。计算机功能分析表明，差异丰度蛋白参与了与宿主防御相关的生物学机制和信号通路，包括病原体识别、直接抗菌功能和急性期反应。该蛋白质组学和生物信息学分析不仅有助于鉴定亚临床乳腺炎的潜在生物标志物，还重现了先前的发现，即乳腺炎感染中宿主防御蛋白的丰度。所有质谱数据均可通过 ProteomeXchange 以标识符 获得。
