pH微电极：改良型托马斯凹尖配置。

pH microelectrode: modified Thomas recessed-tip configuration.

作者信息

Pucacco L R, Corona S K, Jacobson H R, Carter N W

出版信息

Anal Biochem. 1986 Mar;153(2):251-61. doi: 10.1016/0003-2697(86)90089-8.

Abstract

Through the use of a glass-membrane pH electrode and a water-tight seal a modified Thomas pH microelectrode has been developed. The modified Thomas electrode has a relatively low electrical resistance (10(11) omega), a small sensing chamber (10 microns3), and a rapid response time (10 s) and can be manufactured in both single- and double-barreled configurations. The modified Thomas electrode is designed to measure the intracellular pH of small cells such as those found in the mammalian kidney tubule.

摘要

通过使用玻璃膜pH电极和水密密封，开发出了一种改良的托马斯pH微电极。改良的托马斯电极具有相对较低的电阻（10¹¹欧姆）、较小的传感腔（10立方微米）和快速响应时间（10秒），并且可以制成单管和双管配置。改良的托马斯电极旨在测量小细胞的细胞内pH值，如在哺乳动物肾小管中发现的细胞。