体内测量细胞内pH值的快速方法。

Rapid method for measuring intracellular pH in vivo.

作者信息

Calderwood S K, Dickson J A

出版信息

Cell Biol Int Rep. 1978 Jul;2(4):327-37. doi: 10.1016/0309-1651(78)90018-8.

Abstract

Intracellular pH (pHi) was simultaneously measured in 6 normal tissues and a malignant tumour of rats by a rapid triple isotope technique, based on the in vivo distribution of 5,5-dimethyl-2,4-oxazolidinedione-2-14C (DMO), tritiated water and sodium chloride-36. Results compared favourably with pH measured directly in the same rat by capillary glass electrode, and with values of other workers for pHi in rat tissues. Mean pHi of normal tissues was close to pH 7, and in each organ there was a linear relationship between pHi and extracellular pH (pHe) over the normal range of pHe encountered (pH 6.9-7.6). Organ pHi altered in response to administration of NH4Cl or NaHCO3 to the host.

摘要

采用基于5,5-二甲基-2,4-恶唑烷二酮-2-¹⁴C（DMO）、氚水和氯化钠-³⁶在体内分布的快速三重同位素技术，同时测定了大鼠6种正常组织和1个恶性肿瘤的细胞内pH值（pHi）。结果与用毛细管玻璃电极直接在同一只大鼠中测得的pH值以及其他研究人员测得的大鼠组织pHi值相比，情况良好。正常组织的平均pHi接近pH 7，并且在每个器官中，在所遇到的正常细胞外pH值（pHe）范围内（pH 6.9 - 7.6），pHi与pHe之间存在线性关系。向宿主施用氯化铵或碳酸氢钠后，器官pHi发生了变化。