Walash M I, Rizk M, Sheribah Z A, Salim M M
Department of Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Int J Biomed Sci. 2010 Sep;6(3):268-75.
A simple and sensitive spectrofluorimetric method was developed for the determination of biotin in pure form and in pharmaceutical preparations. The proposed method is based on the oxidation of the drug with cerium (IV) ammonium sulfate in acidic medium. The fluorescence of the produced Cerium (III) was measured at 365 nm after excitation at 255 nm. The different experimental parameters affecting the development and stability of the reaction were carefully studied and optimized. The method is applicable over the concentration range of 30-120 ng/mL with correlation coefficient of 0.9998. The detection limit (LOD) of biotin was 2.41 ng/mL while quantitation limit (LOQ) was 7.29 ng/mL. The proposed procedure was successfully applied for the determination of biotin in pharmaceutical preparations with mean recoveries of 99.55 ± 0.83 and 101.67 ± 1.53 for biotin ampoules and capsules, respectively. The results obtained were in good agreement with those obtained using the official method.
开发了一种简单且灵敏的荧光分光光度法，用于测定纯品及药物制剂中的生物素。该方法基于在酸性介质中用硫酸铈（IV）铵氧化药物。在255nm激发后，于365nm处测量生成的铈（III）的荧光。仔细研究并优化了影响反应进程和稳定性的不同实验参数。该方法适用于30 - 120 ng/mL的浓度范围，相关系数为0.9998。生物素的检测限（LOD）为2.41 ng/mL，定量限（LOQ）为7.29 ng/mL。所提出的方法成功应用于药物制剂中生物素的测定，生物素安瓿和胶囊的平均回收率分别为99.55 ± 0.83和101.67 ± 1.53。所得结果与使用官方方法获得的结果高度一致。
